Fabrication of thermo-responsive PNIPAAm-g-ETFE for cell culture dishes by pre-irradiation grafting

被引：6

作者：

Yamahara, Yumi ^{[1
]}

Nagasawa, Naotsugu ^{[2
]}

Taguchi, Mitsumasa ^{[2
]}

Oshima, Akihiro ^{[3
]}

Washio, Masakazu ^{[1
]}

机构：

[1] Waseda Univ, Waseda Res Inst Sci & Engn, Shinjuku Ku, 3-4-1 Okubo, Tokyo 1698555, Japan

[2] Natl Inst Quantum & Radiol Sci & Technol, QuBS, 1223 Watanuki, Takasaki, Gunma 3701292, Japan

[3] Osaka Univ, Grad Sch Engn, 8-1 Mihogaoka, Ibaraki, Osaka 5670047, Japan

来源：

RADIATION PHYSICS AND CHEMISTRY | 2018年 / 142卷

关键词：

ETFE; Cell culture dishes; Pre-irradiation grafting; Thermo-responsive; PNIPPAm-g-ETFE; IRRADIATED POLYTETRAFLUOROETHYLENE; DRUG PERMEATION; PEROXY-RADICALS; TEMPERATURE; COPOLYMER; POLYMERS; ETHYLENE; FILMS; POLY(N-ISOPROPYLACRYLAMIDE); ADHESION;

D O I：

10.1016/j.radphyschem.2017.02.045

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Thermo-responsive templates for the cell cultivation based on Poly(tetrafluoroethylene-co-ethylene) (ETFE) were fabricated by pre-irradiation grafting of N-isoproplyacrylamide (NIPAAm) monomer by electron beam (EB) irradiation under nitrogen gas atmosphere at room temperature, and their characteristic properties were studied. The detachment of cultured HeLa cells from fabricated thermo-responsive templates were attempted. Furthermore, the reaction mechanism is proposed using ESR spectroscopy and FT-IR spectroscopy. It is confirmed that the cultured HeLa cells were detached from fabricated thermo-responsive templates at 20 degrees C. Water contact angle analysis indicated that obtained templates had thermo-response around 30 degrees C. It is suggested that the grafted polymer chains would mainly react with peroxy radicals (-CF2-CF(OO center dot)-) on tetrafluoroethylene unit in ETFE.

引用

页码：88 / 93

页数：6

共 4 条

[1] Role of hyperbranched polystyrene on thermo-responsive cell culture dishes prepared by hyperbranched polystyrene-g-poly(N-isopropylacrylamide)
Sudo, Yu
Sakai, Hideaki
Nabae, Yuta
Hayakawa, Teruaki
Kakimoto, Masa-aki
POLYMER, 2016, 100 : 77 - 85
[2] Preparation of hyperbranched polystyrene-g-poly(N-isopropylacrylamide) copolymers and its application to novel thermo-responsive cell culture dishes
Sudo, Yu
Sakai, Hideaki
Nabae, Yuta
Hayakawa, Teruaki
Kakimoto, Masa-aki
POLYMER, 2015, 70 : 307 - 314
[3] Design of thermo-responsive cell culture dishes using poly (N-isopropylacrylamide)-block-polystyrene copolymers for cell sheet technology
Hajibabazadeh, Sina
Ghaleh, Hakimeh
Abbasi, Farhang
Foroutani, Kamran
EUROPEAN POLYMER JOURNAL, 2023, 195
[4] Fabrication of thermo-responsive cell-culture membranes with Poly(N-isopropylacrylamide) by electron-beam graft polymerization
Shimura, Ryoya
Suematsu, Yoshitaka
Horiuchi, Hiroto
Takeoka, Shinji
Oshima, Akihiro
Washio, Masakazu
RADIATION PHYSICS AND CHEMISTRY, 2020, 171

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