Determination of the degree of N-acetylation (DA) of chitin and chitosan in the presence of water by first derivative ATR FTIR spectroscopy

被引：47

作者：

Beil, Stephan ^{[1
]}

Schamberger, Anna ^{[1
]}

Naumann, Wolfgang ^{[1
]}

Machill, Susanne ^{[1
]}

van Pee, Karl-Heinz ^{[1
]}

机构：

[1] Tech Univ Dresden, D-01062 Dresden, Germany

来源：

CARBOHYDRATE POLYMERS | 2012年 / 87卷 / 01期

关键词：

Chitosan; Chitin; Degree of N-acetylation; First derivative ATR FTIR spectroscopy; INFRARED-SPECTROSCOPY; DEACETYLATION; SPECTROPHOTOMETRY; SAMPLES;

D O I：

10.1016/j.carbpol.2011.07.025

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A new method for the determination of the degree of N-acetylation (DA) of chitin and chitosan is described using first derivative diamond ATR FTIR spectroscopy. Applying the derivative values of the amide Ill band at 1327 cm(-1) and the CH deformation band of the N-acetyl group at 1383 cm(-1) as measure of the N-acetyl content of the sample in relation to the derivative value of the bridge oxygen vibration at 1163 cm(-1) as internal standard, a linear correlation to the results of first derivative UV spectroscopy was obtained and confirmed by elemental analysis and Raman spectroscopy. The described method allows the determination of the degree of N-acetylation of chitosan and chitin in the presence of water thus making drying procedures unnecessary. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：117 / 122

页数：6

共 19 条

[1] DETERMINATION OF THE DEGREE OF N-ACETYLATION OF CHITIN CHITOSAN WITH PICRIC ACID
NEUGEBAUER, WA
NEUGEBAUER, E
BRZEZINSKI, R
[J]. CARBOHYDRATE RESEARCH, 1989, 189 : 363 - 367
[2] Determination of the degree of N-acetylation for chitin and chitosan by various NMR spectroscopy techniques: A review
Kasaai, Mohammad R.
[J]. CARBOHYDRATE POLYMERS, 2010, 79 (04) : 801 - 810
[3] Determination of the degree of acetylation (DA) of chitin and chitosan by an improved first derivative UV method
Wu, Tao
Zivanovic, Svetlana
[J]. CARBOHYDRATE POLYMERS, 2008, 73 (02) : 248 - 253
[4] Various Methods for Determination of the Degree of N-Acetylation of Chitin and Chitosan: A Review
Kasaai, Mohammad R.
[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2009, 57 (05) : 1667 - 1676
[5] DETERMINATION OF THE DEGREE OF N-ACETYLATION OF CHITOSAN
MOORE, GK
ROBERTS, GAF
[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1980, 2 (02) : 115 - 116
[6] Determination of N-acetylation degree in chitosan using Raman spectroscopy
Zajac, A.
Hanuza, J.
Wandas, M.
Dyminska, L.
[J]. SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2015, 134 : 114 - 120
[7] Straightforward determination of the degree of N-acetylation of chitosan by means of first-derivative UV spectrophotometry
da Silva, Ricardo M. P.
Mano, Jado F.
Reis, Rui L.
[J]. MACROMOLECULAR CHEMISTRY AND PHYSICS, 2008, 209 (14) : 1463 - 1472
[8] An optimised method to determine the degree of acetylation of chitin and chitosan by FTIR spectroscopy
Duarte, ML
Ferreira, MC
Marvao, MR
Rocha, J
[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2002, 31 (1-3) : 1 - 8
[9] A review of several reported procedures to determine the degree of N-acetylation for chitin and chitosan using infrared spectroscopy
Kasaal, Mohammad R.
[J]. CARBOHYDRATE POLYMERS, 2008, 71 (04) : 497 - 508
[10] IMPROVED METHOD FOR IR DETERMINATION OF THE DEGREE OF N-ACETYLATION OF CHITOSAN
BAXTER, A
DILLON, M
TAYLOR, KDA
ROBERTS, GAF
[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1992, 14 (03) : 166 - 169

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