Objective: The research tried to create a questionnaire with which we can measure effectively and investigate features of the academic stressors of college students. Methods: In this paper, aiming at the academic stressors of college student in study, we completed college student' s academic stressors questionnaire based on the literature study, theory discussion, open questionnaire and depth interview. Results: (1)The questionnaire consisted of eight factors which included prospects stress, achievement goal stress, task requires stress, competition stress, setbacks stress, parents stress, expect stress and environmental stress. Their contribution rate were 8.615, 6.989, 6.789, 6.656, 6.508, 5.740, 5.712 and 4.921 respectively. (2)The Cronbach alpha coefficients of the questionnaire was 0.9577. The split-half reliability was 0.9250. The correlation coefficients among these factors ranged from 0.361 to 0.613. The correlation coefficients of these factors with the total question score ranged from 0.606 to 0.852. (3)The result of the confirmatory factor analysis shown that the fitting indexes which included RMSEA(the RMSEA value is 0.042), NFI(0.836), CFI(0.915), GFI(0.862) and IFI(0.916) achieved the requirement of psychometrics. Conclusion: Development of the academic stressors questionnaire for college student could be widely applied to test academic stressors of university.