The enhanced electrogenerated chemiluminescence of Ru(bpy)32+ by glutathione on a glassy carbon electrode modified with some porphine compounds

It has been found that the electrochemical activity of glutathione was increased greatly at the glassy carbon electrodes modified with 5,10,15,20-tetraphenylporphine ruthenium(ii) carbenyl (RuTPP), meso-tetraphenylporphine copper(ii) complex (CuTTP) and hemin. It has been also found that glutathione would enhance the electrogenerated chemiluminescence (ECL) of Ru(bpy)(3)(2+) at a hemin glassy carbon electrode; the enhanced ECL intensity was linear with the concentration of glutathione in the range of 1 x 10(-7) similar to 1 x 10(-4) mol l(-1), based on which method for determination of glutathione has been developed. The detection limit of glutathione was 2 x 10(-8) mol l(-1), and the relative standard deviation for 1 x 10(-6) mol l(-1) glutathione was 2.7%. The mechanism for this ECL system has been proposed.