Crystallization and preliminary crystallographic analysis of endo-1,3-β-glucanase from Arthrobacter sp.

被引：5
|
作者：
Pang, Z
Kang, YN
Ban, M
Oda, M
Kobayashi, R
Ohnishi, M
Mikami, B
机构：
[1] Kyoto Prefectural Univ, Grad Sch Agr, Sakyo Ku, Kyoto 6068522, Japan
[2] Kyoto Univ, Grad Sch Agr, Lab Food Qual Design & Dev, Uji, Kyoto 6110011, Japan
来源：
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS | 2005年 / 61卷
关键词：
D O I：
10.1107/S1744309104027915
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Endo-1,3-beta-glucanases hydrolyze internal 1,3-beta-glucosyl linkages. The endo-1,3-beta- glucanase from Arthrobacter sp. was crystallized by the hanging-drop vapour-diffusion method. The crystals belonged to space group P4(1), with unit-cell parameters a = 71.31, c = 60.07 angstrom, and contained one molecule per asymmetric unit. The Matthews coefficient (V-M) and the solvent content were 2.35 angstrom(3) Da(-1) and 47.63%, respectively. Diffraction data were collected to a resolution of 1.66 angstrom at SPring-8 using a MAR CCD area detector and gave a data set with an overall R-merge of 5.4% and a completeness of 99.4%.
引用
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页码：68 / 70
页数：3
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