Synthesis of CID-cleavable protein crosslinking agents containing quaternary amines for structural mass spectrometry

被引：6

作者：

Hagen, Susan E. ^{[1
]}

Liu, Kun ^{[1
]}

Jin, Yafei ^{[1
]}

Piersimoni, Lolita ^{[2
]}

Andrews, Philip C. ^{[2
,3
,4
]}

Showalter, Hollis D. ^{[1
]}

机构：

[1] Univ Michigan, Coll Pharm, Dept Med Chem, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Dept Biol Chem, Med Sch, Ann Arbor, MI 48109 USA

[3] Univ Michigan, Dept Bioinformat, Med Sch, Ann Arbor, MI 48109 USA

[4] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2018年 / 16卷 / 37期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1039/c8ob00329g

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Two novel cyclic quaternary amine crosslinking probes are synthesized for structural mass spectrometry of protein complexes in solution and for analysis of protein interactions in organellar and whole cell extracts. Each exhibits high aqueous solubility, excellent protein crosslinking efficiencies, low collision induced dissociation (CID) energy fragmentation efficiencies, high stoichiometries of reaction, increased charges of crosslinked peptide ions, and maintenance of overall surface charge balance of crosslinked proteins.

引用

页码：8245 / 8248

页数：4

共 13 条

[1] Design of CID-cleavable protein cross-linkers: identical mass modifications for simpler sequence analysis
Kandur, Wynne V.
Kao, Athit
Vellucci, Danielle
Huang, Lan
Rychnovsky, Scott D.
[J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2015, 13 (38) : 9793 - 9807
[2] Quaternary Diamines as Mass Spectrometry Cleavable Crosslinkers for Protein Interactions
Clifford-Nunn, Billy
Showalter, H. D. Hollis
Andrews, Philip C.
[J]. JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2012, 23 (02) : 201 - 212
[3] Performing protein crosslinking using gas-phase cleavable chemical crosslinkers and liquid chromatography-tandem mass spectrometry
Argo, Andrew S.
Shi, Chunxiao
Liu, Fan
Goshe, Michael B.
[J]. METHODS, 2015, 89 : 64 - 73
[4] A Unified Workflow to Identify Quaternary Structures and Changes in Protein-protein Interactions from Crosslinking-based Mass Spectrometry
Barnier, Catherine
Piersimoni, Lolita
Price, Janet
Plasencia, Manolo
Chapman, Matthew R.
Andrews, Phillip C.
Freddolino, Peter L.
[J]. BIOPHYSICAL JOURNAL, 2020, 118 (03) : 357A - 357A
[5] CHEMICAL SURFACE MODIFICATION AND CHEMICAL CROSSLINKING COMBINED WITH MASS SPECTROMETRY FOR PROTEIN TERTIARY STRUCTURAL INFORMATION
Deterding, Leesa J.
Tomer, Kenneth B.
[J]. APPLICATIONS OF MASS SPECTROMETRY IN LIFE SAFETY, 2008, : 139 - 150
[6] Synthesis of two new enrichable and MS-cleavable cross-linkers to define protein-protein interactions by mass spectrometry
Burke, Anthony M.
Kandur, Wynne
Novitsky, Eric J.
Kaake, Robyn M.
Yu, Clinton
Kao, Athit
Vellucci, Danielle
Huang, Lan
Rychnovsky, Scott D.
[J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2015, 13 (17) : 5030 - 5037
[7] Synthesis and structural investigation of a series of mannose-containing oligosaccharides using mass spectrometry
Daikoku, S.
Pendrill, R.
Kanie, Y.
Ito, Y.
Widmalm, G.
Kanie, O.
[J]. ORGANIC & BIOMOLECULAR CHEMISTRY, 2018, 16 (02) : 228 - 238
[8] Track: Integrating Techniques to Address Challenges in Protein Structural Biology Proteome-Wide Profiling of Protein Misfolding with Crosslinking Mass Spectrometry
Xia, Yingzi
Fried, Stephen
[J]. PROTEIN SCIENCE, 2023, 32
[9] An acidic residue reactive and disulfide bond-containing cleavable crosslinker for probing protein 3D structures based on electrochemical mass spectrometry
Cui, Lili
Ma, Yongge
Li, Ming
Wei, Zhonglin
Huan, Yanfu
Fei, Qiang
Li, Hongmei
Zheng, Lianyou
[J]. TALANTA, 2020, 216
[10] Integration of online digestion and electrolytic reduction with mass spectrometry for rapid disulfide-containing protein structural analysis
Zheng, Qiuling
Zhang, Hao
Chen, Hao
[J]. INTERNATIONAL JOURNAL OF MASS SPECTROMETRY, 2013, 353 : 84 - 92

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