Methylation profiling of mesothelioma using real-time methylation-specific PCR: A pilot study

被引:8
|
作者
Pu, Robert T.
Sheng, Zong-Mei
Michael, Claire W.
Rhode, Michael G.
Clark, Douglas P.
O'Leary, Timothy J.
机构
[1] Univ Michigan, Sch Med, Dept Pathol, Ann Arbor, MI 48109 USA
[2] Armed Forces Inst Pathol, Dept Cellular Pathol & Genet, Rockville, MD USA
[3] Johns Hopkins Med Inst, Dept Pathol, Baltimore, MD 21205 USA
[4] Vet Hlth Adm, Washington, DC USA
关键词
mesothelioma; gene methylatio profiling; real-time MSP;
D O I
10.1002/dc.20692
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
We tested whether methylation profiles generated by real-time methylation-specific PCR (MSP) can be useful in differentiating benign, reactive mesothelial cell proliferation (RM) from malignant mesothelioma (MM). Forty-two of the 63 cases (67%) yielded informative results for RAR beta 2, GPC3, CDKN2A (p16), TERT, and CCND2 (cyclinD2) gene methylation. DNA methylation of any gene was observed in much higher frequency in MM cases than RM cases (63% vs. 33%, P < 0.05). Individual gene methylation was higher in the MM than the RM cases for most of the genes; however, this was not statistically significant (RAR beta 2: 58% vs. 33%, P > 0.05; GPC3: 36% vs. 27%, P > 0.05; CDKN2A: 4% vs. 0%; TERT: 4% vs. 0%), while CCND2 methylation was not detected in any case. Although preliminary, we demonstrate that real-time MSP can be applied to archival specimens and gene methylation profiling may have potential to be a useful ancillary tool to help distinguish MM front RM.
引用
收藏
页码:498 / 502
页数:5
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