Lipid-protein nanoscale bilayers: A versatile medium for NMR investigations of membrane proteins and membrane-active peptides

被引：50

作者：

Lyukmanova, Ekaterina N. ^{[1
]}

Shenkarev, Zakhar O. ^{[1
]}

Paramonov, Alexander S. ^{[1
]}

Sobol, Alexander G. ^{[1
]}

Ovchinnikova, Tatiana V. ^{[1
]}

Chupin, Vladimir V. ^{[1
]}

Kirpichnikov, Mikhail P. ^{[1
]}

Blommers, Marcel J. J. ^{[2
]}

Arseniev, Alexander S. ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117997, Russia

[2] Novartis Inst BioMed Res, CH-4002 Basel, Switzerland

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 07期

关键词：

D O I：

10.1021/ja0777988

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In the present Communication we demonstrate the possibility to use high-resolution NMR for the investigation of membrane proteins in reconstituted high-density lipoprotein (rHDL) particles. The rHDL particles are nanoscale phospholipid bilayers wrapped around by a dimer of apolipoprotein A-1 (Bayburt, T. H.; Grinkova, Y. V.; Sligar, S. G. Nano Left. 2002, 2, 853-856). In contrast to the commonly used spherical micelles, the rHDL particles incorporate a lipid bilayer like in biological membranes. These particles still undergo isotropic motion on the NMR time scale, providing the application of high-resolution NMR spectroscopy of the peptides and proteins embedded into their bilayer. As an example, the topology of the membrane-active peptide Antiamoebin-I in the bilayer of the rHDL particles was determined by using the lipid-soluble relaxation probe technique.

引用

页码：2140 / +

页数：3

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