Identification of anthracene degraders in leachate-contaminated aquifer using stable isotope probing

被引:29
|
作者
Zhang, Shuying [1 ]
Wan, Rui [1 ]
Wang, Qingfeng [1 ]
Xie, Shuguang [1 ]
机构
[1] Peking Univ, Coll Environm Sci & Engn, Beijing 100871, Peoples R China
基金
中国国家自然科学基金;
关键词
Polycyclic aromatic hydrocarbons (PAHs); Stable isotope probing (SIP); Groundwater; Aquifer; Biodegradation; Variovoraxs; POLYCYCLIC AROMATIC-HYDROCARBONS; BIODEGRADATION; SOIL; PAH; BACTERIA; PHENANTHRENE; DEGRADATION; NAPHTHALENE; CONSORTIUM; MICROCOSMS;
D O I
10.1016/j.ibiod.2011.10.002
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Polycyclic aromatic hydrocarbons (PAHs) are common contaminants in landfill leachate-contaminated aquifer. It is necessary to identify the microorganisms truly responsible for PAH degradation if bioremediation can be applied as an effective technology. DNA-based stable isotope probing (SIP) in combination with terminal restriction fragment length polymorphism (TRFLP) was used to identify the active anthracene degraders in the contaminated aquifer sediment One kind of degrader was classified as Variovorax species within class beta-proteobacteria, but another belonged to unclassified bacteria. These findings also suggest novel microorganisms involved in PAH-degrading processes. (C) 2011 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1224 / 1228
页数:5
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