Visual and real-time imaging focusing for highly sensitive laser-induced fluorescence detection at yoctomole levels in nanocapillaries

被引：14

作者：

Zhang, Wenmei ^{[1
]}

Liu, Lei ^{[1
]}

Zhang, Qi ^{[1
]}

Zhang, Dongtang ^{[1
]}

Hu, Qin ^{[1
]}

Wang, Yanan ^{[1
]}

Wang, Xiayan ^{[1
]}

Pu, Qiaosheng ^{[2
]}

Guo, Guangsheng ^{[1
,3
]}

机构：

[1] Beijing Univ Technol, Beijing Key Lab Green Catalysis & Separat, Dept Chem & Chem Engn, Ctr Excellence Environm Safety & Biol Effects, Beijing 100124, Peoples R China

[2] Lanzhou Univ, Dept Chem, Lanzhou 730000, Gansu, Peoples R China

[3] Beijing Acad Sci & Technol, Beijing 100089, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2020年 / 56卷 / 16期

基金：

中国国家自然科学基金;

关键词：

SOLUTION HYDRODYNAMIC SEPARATION; LIQUID-CHROMATOGRAPHY; OPEN CAPILLARIES; NARROW; DNA; SYSTEM; SPECTROSCOPY;

D O I：

10.1039/c9cc09594b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A highly sensitive laser-induced fluorescence detection system with real-time imaging focusing instead of the use of fluorescent reagents were developed for the detection of analytes in nanocapillaries. A cylindrical lens was adopted for the shaping of a laser beam to increase its spatial resolution. The limit of detection with a capillary having a radius of 440 nm was 6.76 yoctomoles (or four molecules) for fluorescein, and 84.5 yoctomoles (or 51 molecules) for FAM-labeled Hsa-miR-17. The designed system exhibited high sensitivity and stability for femtoliters of sample and was hence indicated to be suitable for analyses of single cells.

引用

页码：2423 / 2426

页数：4

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