Identification of Sterol-Containing Domains in Vacuolar Membranes by means of Confocal Microscopy

被引：0

作者：

Nurminsky, V. N. ^{[1
]}

Nesterkina, I. S. ^{[1
]}

Spiridonova, E. V. ^{[1
]}

Rakevich, A. L. ^{[2
]}

Ozolina, N. V. ^{[1
]}

机构：

[1] Russian Acad Sci, Siberian Branch, Siberian Inst Plant Physiol & Biochem, Ul Lermontova 132, Irkutsk 664033, Russia

[2] Russian Acad Sci, Siberian Branch, Irkutsk Dept, Inst Laser Phys, Ul Lermontova 130a, Irkutsk 664033, Russia

来源：

BIOLOGICHESKIE MEMBRANY | 2017年 / 34卷 / 04期

关键词：

vacuolar membrane; lipid-protein microdomains; rafts; sterols; confocal microscopy; Filipin; LIPID RAFTS; PLASMA-MEMBRANE; CHOLESTEROL; PROBE; MICRODOMAINS; ORGANIZATION; FILIPIN; CELLS;

D O I：

10.7868/S0233475517040065

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The method of confocal microscopy and membrane probe Filipin were applied for the identification of sterol-containing domains in the tonoplast of beet root cells. The presence of sterol-containing domains in the tonoplast was confirmed using methyl-beta-cyclodextrin. It was found that sterol-containing vacuolar membrane included separate small sterol-enriched sites with a more intensive fluorescence; these sites can be attributed to raft structures. Our data demonstrate that the sterol content in isolated rafts can be assessed by confocal microscopy. A detailed analysis of the isolated rafts indicated that they contain (beta-sitosterol, stigmasterol, alpha-tocopherol, and cholesterol.

引用

页码：307 / 312

页数：6

共 9 条

[1] Identification of sterol-containing domains in vacuolar membranes by confocal microscopy
Nurminsky V.N.
Nesterkina I.S.
Spiridonova E.V.
Rakevich A.L.
Ozolina N.V.
Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology, 2017, 11 (4) : 296 - 300
[2] Effects of Flavonoids on Dipole Potential of Sterol-Containing Membranes
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[6] Physical Properties of Lipid Membranes Containing Sterol Studied by Deuterium NMR and Fluorescence Microscopy
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[7] Blue-black rule: identification of histologic substrate by means of in vivo confocal microscopy
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Longo, C.
Argenziano, G.
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JOURNAL OF INVESTIGATIVE DERMATOLOGY, 2012, 132 : S134 - S134
[8] Identification of traction-separation parameters by means of peel testing and in situ confocal microscopy
Cattarinuzzi, Emanuele
Gastaldi, Dario
Vena, Pasquale
Pozzobon, Fiorella
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JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2019, 29 (03)
[9] Identification of immunological reagents for use in the study of freshwater planarians by means of whole-mount immunofluorescence and confocal microscopy
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GENESIS, 2002, 32 (04) : 293 - 298

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