Spherical aberration correction in nonlinear microscopy and optical ablation using a transparent deformable membrane

被引：45
|
作者：
Tsai, P. S.
Migliori, B.
Campbell, K.
Kim, T. N.
Kam, Z.
Groisman, A. [1 ]
Kleinfeld, D.
机构：
[1] Univ Calif San Diego, Dept Phys, La Jolla, CA 92093 USA
[2] Weizmann Inst Sci, IL-761002 Rehovot, Israel
[3] Univ Calif San Diego, Grad Program Neurosci, La Jolla, CA 92093 USA
来源：
APPLIED PHYSICS LETTERS | 2007年 / 91卷 / 19期
基金：
美国国家科学基金会; 美国国家卫生研究院;
关键词：
D O I：
10.1063/1.2804014
中图分类号：
O59 [应用物理学];
学科分类号：
摘要：
We describe the design and utilization of a deformable membrane to minimize the negative spherical aberration that occurs when a standard water-dipping objective is used to focus within a higher-index sample. In connection with two-photon laser scanning microscopy, we demonstrate twofold improved axial resolution of structures as deep as 1 mm in gels and brain tissue. In conjunction with plasma-mediated ablation, we demonstrate enhanced production of optical damage deep within a glass substrate. The present method provides a simple and inexpensive correction for a limited yet important class of optical aberrations.
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页数：3
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