Use of Scopulariopsis acremonium for the production of cellulase and xylanase through submerged fermentation

被引：0

作者：

Sarao, Loveleen Kaur ^{[1
]}

Arora, Maninder ^{[1
]}

Sehgal, V. K. ^{[1
]}

机构：

[1] Punjab Agr Univ, Dept Proc & Food Engn, Ludhiana 141004, Punjab, India

来源：

AFRICAN JOURNAL OF MICROBIOLOGY RESEARCH | 2010年 / 4卷 / 14期

关键词：

Scopulariopsis acremonium; cellulase; xylanase; submerged fermentation; FUNGAL;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Production of cellulase and xylanase by Scopulariopsis acremonium through submerged fermentation was studied using shake flask cultivation media. The effect of process parameters such as effect of temperature, pH and inoculum size was investigated. The enzymes were partially purified using isopropanol and used for enzyme assays. The results indicated that the maximum cellulase and xylanase having an enzyme activity of 694.45 and 931.25 IU, respectively, were produced at 30 degrees C incubation temperature. The pH optimum to achieve these enzyme activities was 5.5 with an inoculum size of 1 x 10(5) spores ml(-1) of tween - 80. Cellulase was deactivated at 80 degrees C while xylanase was deactivated at 70 degrees C.

引用

页码：1506 / 1509

页数：4

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