Flavobacterium terriphilum sp nov., isolated from soil

A novel aerobic, yellow-pigmented, Gram-stain-negative, rod-shaped bacterial strain, CUG00004(T), was isolated from a soil sample. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CUG00004(T) was a member of the genus Flavobacterium and showed high sequence similarity with Flavobacterium soli DSM 19725(T) (96.9 %) and Flavobacterium glaciei CGMCC 0499(T) (95.6 %). The level of DNA-DNA relatedness between strain CUG00004(T) and F. soli DSM 19725(T) and F. glaciei CGMCC 1.5380(T) was 42.5 and 43.4 %, respectively. Strain CUG00004(T) was moderately alkaliphilic and grew optimally at pH 8.0, at 28 degrees C and in the presence of 0-1%(w/v) NaCl. This organism contained menaquinone-6 (MK-6) as the only isoprenoid quinone and iso-C-15:0, iso-C-17:0 3-OH, anteiso-C-15:0 and summed feature 9 (comprising iso-C-17:1 omega 9c and/or C-16:0 10-methyl) as the major fatty acids. The DNA G+C content of strain CUG00004(T) was 36.3 mol%. On the basis of phylogenetic, physiological and chemotaxonomic analyses, strain CUG00004(T) represents a novel species, for which the name Flavobacterium terriphilum sp. nov. is proposed. The type strain is CUG00004(T) (=CCTCC AB 2014151(T) = KCTC 42876(T)).