We examined whether daily hot water immersion (HWI) after exercise in temperate conditions induces heat acclimation and improves endurance performance in temperate and hot conditions. Seventeen non-heat-acclimatized males performed a 6-day intervention involving a daily treadmill run for 40min at 65% V.O-2max in temperate conditions (18 degrees C) followed immediately by either HWI (N=10; 40 degrees C) or thermoneutral (CON, N=7; 34 degrees C) immersion for 40min. Before and after the 6-day intervention, participants performed a treadmill run for 40min at 65% V.O-2max followed by a 5-km treadmill time trial (TT) in temperate (18 degrees C, 40% humidity) and hot (33 degrees C, 40% humidity) conditions. HWI induced heat acclimation demonstrated by lower resting rectal temperature (T-re, mean, -0.27 degrees C, P<0.01), and final T-re during submaximal exercise in 18 degrees C (-0.28 degrees C, P<0.01) and 33 degrees C (-0.36 degrees C, P<0.01). Skin temperature, T-re at sweating onset and RPE were lower during submaximal exercise in 18 degrees C and 33 degrees C after 6days in HWI (P<0.05). Physiological strain and thermal sensation were also lower during submaximal exercise in 33 degrees C after 6days in HWI (P<0.05). HWI improved TT performance in 33 degrees C (4.9%, P<0.01) but not in 18 degrees C. Thermoregulatory measures and performance did not change in CON. Hot water immersion after exercise on 6days presents a simple, practical, and effective heat acclimation strategy to improve endurance performance in the heat.