HPTLC method for determination of acteoside in ribwort plantain (Plantago lanceolata L.)

被引：6
|
作者：
Umek, A [1 ]
Rupert, A [1 ]
Mlinaric, A [1 ]
Kac, J [1 ]
机构：
[1] Fac Pharm, SI-1000 Ljubljana, Slovenia
来源：
JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC | 2005年 / 18卷 / 102期
关键词：
Plantago lanceolata; acteoside; verbascoside; phenylpropanoid glycosides; HPTLC;
D O I：
10.1556/JPC.18.2005.2.10
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A sensitive thin layer chromatographic method has been established for quantification of acteoside in the leaves of Plantago lanceolata. Chromatographic separation was performed on silica gel HPTLC plates with ethyl acetate-formic acid-water, 18 + 1 + 1 (v/v), as mobile phase. The plates were scanned densitometrically at lambda = 334 nm. The method was validated for precision, repeatability, and accuracy. It was found to be precise - intra-day and inter-day RSD were 0.58 and 2.0%, respectively. Instrumental precision and repeatability of the method were found to be 0.62 and 1.5 (%CV), respectively. The accuracy was checked by studying recovery at three levels; average recovery was 102.3%. The method was used for estimation of acteoside in the leaves of Plantago lanceolata and was confirmed to be suitable for the determination of these compounds in the drug.
引用
收藏
页码：147 / 150
页数：4
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