Probing the Active Site of an O2-Tolerant NAD+-Reducing [NiFe]-Hydrogenase from Ralstonia eutropha H16 by In Situ EPR and FTIR Spectroscopy

被引：52
|
作者：
Horch, Marius [2 ]
Lauterbach, Lars [1 ]
Saggu, Miguel [2 ]
Hildebrandt, Peter [2 ]
Lendzian, Friedhelm [2 ]
Bittl, Robert [3 ]
Lenz, Oliver [1 ]
Zebger, Ingo [2 ]
机构：
[1] Humboldt Univ, Inst Biol Mikrobiol, Sekr PC14, D-10115 Berlin, Germany
[2] Tech Univ Berlin, Inst Chem, D-10623 Berlin, Germany
[3] Free Univ Berlin, Inst Phys, D-10115 Berlin, Germany
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 43期
关键词：
biocatalysis; EPR spectroscopy; FTIR spectroscopy; hydrogenases; oxygen tolerance; NAD-REDUCING HYDROGENASE; VULGARIS MIYAZAKI-F; NIFE HYDROGENASE; OXYGEN; ACTIVATION; OXIDATION; STATE; INSIGHTS; HYSCORE; ENDOR;
D O I：
10.1002/anie.201002197
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A clear picture: In situ EPR and FTIR spectroscopic studies on the soluble, NAD+-reducing [NiFe]-hydrogenase of Ralstonia eutropha reveal that the catalytic site resides predominantly in the intermediate Nia-C state within whole cells. This state, can either be reversibly oxidized to a Nir-B-like state or further reduced to various Nia-SR species. The data suggest that the iron center in the active site contains a standard set (one CO and two CN-) of inorganic ligands. © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
引用
收藏
页码：8026 / 8029
页数：4
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