Target class strategies in mass spectrometry-based proteomics

被引:8
|
作者
Opiteck, GJ
Scheffler, JE
机构
[1] Ortho Biotech LP, New Business Dev, Bridgewater, NJ 08807 USA
[2] Bristol Myers Squibb Co, Clin Discovery Technol, Princeton, NJ 08543 USA
关键词
affinity purification; biomarker; LC/MS; mass spectrometry; proteomics; target class;
D O I
10.1586/14789450.1.1.57
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The cornerstone of proteomics resides In using traditional methods of protein chemistry, to extract and resolve complex mixtures, in concert with the powerful engines of mass spectrometry to decipher peptide and protein identities. The broad utility of proteomics technologies to map protein interactions, understand regulatory mechanisms and identify biomarkers associated with disease states and drug treatments necessitates a targeted biochemical approach tailored to the characteristics of the tissue, fluid or cellular extract being studied. The application of affinity methods in proteomic studies to focus on particular classes of molecules is being used with increasing frequency and comprises the subject of this review. An overview of successfully applied affinity methods is provided, along with speculation on the use of innovative approaches. Sample preparation and processing are critical for proteomics with affinity reagents, as only functional and active proteins can be isolated in most cases. Considerations for methods of sample preparation to optimize affinity capture and release are also discussed.
引用
收藏
页码:57 / 66
页数:10
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