Binding of proflavin to chymotrypsin: an experiment to determine protein-ligand interactions by direct nonlinear regression analysis of spectroscopic titration data

被引：0
|
作者：
Hendrickson, HS [1 ]
机构：
[1] Univ Washington, Dept Chem, Seattle, WA 98195 USA
来源：
BIOCHEMICAL EDUCATION | 1999年 / 27卷 / 02期
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D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The dissociation constant and stoichiometry of a proflavin-chymotrypsin complex are determined by spectroscopic titration and direct nonlinear regression data analysis in a simple experiment during one laboratory period. (C) 1999 IUBMB. Published by Elsevier Science Ltd. All rights reserved.
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页码：118 / 121
页数：4
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