Spectroscopic Studies on the Toxic Interaction of Sodium Oleate with Bovine Serum Albumin

被引：2
|
作者：
Fang, Xiaoyan [1 ]
Liu, Chuan [1 ]
Liu, Rutao [1 ]
Teng, Yue [1 ]
机构：
[1] Shandong Univ, Shandong Key Lab Water Pollut Control & Resource, Sch Environm Sci & Engn, China Amer CRC Environm & Hlth, Jinan 250100, Shandong, Peoples R China
来源：
JOURNAL OF FLUORESCENCE | 2011年 / 21卷 / 03期
关键词：
Sodium oleate; Bovine serum albumin; Spectroscopy; Interaction; FLUORESCENCE; BINDING; ACID; SURFACTANTS; HEMOGLOBIN; RESIDUES; COMPLEX; PROBE;
D O I：
10.1007/s10895-010-0779-x
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The effects of sodium oleate on Bovine Serum Albumin (BSA) were investigated by fluorescence, synchronous fluorescence, ultraviolet-visible and circular dichroism spectroscopy. According to the experiment results, we found that the fluorescence intensity of BSA was quenched by sodium oleate following a static mode, a sodium oleate-BSA complex was formed and the binding site was calculated approximately equal to 1. The experimental results showed that the hydrophilic group (-COONa) can bind to the BSA and lead to the looser of the protein conformation, the microenvironment and the secondary structure elements were changed in the presence of sodium oleate. This work reflected the toxic interaction mechanism of BSA and sodium oleate from the perspective of spectroscopy.
引用
收藏
页码：1069 / 1074
页数：6
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