Measuring cell density in a acoustofluidic micro-cavity

被引：0
|
作者：
Santos, Harrisson D. A. [1 ]
Silva, Giclenio C. [1 ]
Vieira, Tiago F. [2 ]
Silva, Amanda E. [3 ]
de Araujo, Icaro B. Q. [2 ]
Jacinto, Carlos [4 ]
Rocha, Ueslen [4 ]
Alexandre-Moreira, Magna S. [3 ]
Silva, Glauber T. [1 ]
机构：
[1] Univ Fed Alagoas, Phys Acoust Grp, Maceio, Alagoas, Brazil
[2] Univ Fed Alagoas, Inst Comp, Maceio, Alagoas, Brazil
[3] Univ Fed Alagoas, Lab Pharmacol, Maceio, Alagoas, Brazil
[4] Univ Fed Alagoas, Nanophoton & Images Grp, Maceio, Alagoas, Brazil
来源：
2021 IEEE UFFC LATIN AMERICA ULTRASONICS SYMPOSIUM (LAUS) | 2021年
关键词：
TORQUE;
D O I：
10.1109/LAUS53676.2021.9639224
中图分类号：
O42 [声学];
学科分类号：
070206 ; 082403 ;
摘要：
Detecting changes in the density of biological cells is of paramount importance as it may reveal the cell status in processes such as differentiation and disease. Several methods have been devised to measure cell density in microfluidic devices. Here, we introduce a 3D printed microfluidic wherein cells are levitated by means of the acoustic forces of ultrasonic waves. The density can be inferred by measuring the height versus time of a single cell in free fall. We determine the density of 10 mu m-polystyrene beads and macrophage cells (line j774.A1) with high accuracy.
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页数：4
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