Differential effects of butyrate and dibutyryl cAMP on mRNA levels of muscarinic acetylcholine receptor subtypes expressed in neurohybrid cell lines

被引：3

作者：

Fukamauchi, F

Mataga, N

Wang, YJ

Chuang, DM

机构：

[1] NIMH,BIOL PSYCHIAT BRANCH,MOLEC NEUROBIOL SECT,BETHESDA,MD 20892

[2] TOKYO MED & DENT UNIV,MED RES INST,DEPT MOL MED SCI,CHIYODA KU,TOKYO 101,JAPAN

来源：

NEUROSCIENCE LETTERS | 1996年 / 212卷 / 01期

关键词：

muscarinic acetylcholine receptor mRNA; butyrate; dibutyryl cAMP; NCB-20; NG108-15; northern blot analysis;

D O I：

10.1016/0304-3940(96)12779-8

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

NCB-20 cells expressed m1- and m4-muscarinic acetylcholine receptor (mAChR) mRNAs, while NG108-15 cells expressed only m4-mAChR mRNA. Butyrate induced a time-dependent increase in the level of m1-mAChR mRNA with no change in the m4-mAChR mRNA level in NCB-20 cells. Similarly, butyrate did not affect the m4-mAChR mRNA level in NG108-15 cells. In contrast, dibutyryl cAMP caused a significant time-dependent decrease in the level of m4-mAChR mRNA in NCB-20 and NG108-15 cells as well as m1-mAChR mRNA in NCB-20 cells. Our results suggest that these two differentiating agents are important physiological regulators of the transcription and/or stability of the mRNA of certain mAChR subtypes expressed in these two neurohybrid cell lines.

引用

页码：49 / 52

页数：4

共 11 条

[1] Ethanol induces subtype-specific up-regulation of muscarinic acetylcholine receptor mRNA in neurohybrid cell lines
Fukamauchi, F
Wang, YJ
Chuang, DM
LIFE SCIENCES, 1997, 62 (05) : 389 - 396
[2] Diversity of mRNA expression for muscarinic acetylcholine receptor subtypes and neuronal nicotinic acetylcholine receptor subunits in human mononuclear leukocytes and leukemic cell lines
Sato, KZ
Fujii, T
Watanabe, Y
Yamada, S
Ando, T
Kazuko, F
Kawashima, K
NEUROSCIENCE LETTERS, 1999, 266 (01) : 17 - 20
[3] Effects of organophosphate exposure on muscarinic acetylcholine receptor subtype mRNA levels in the adult rat
Yagle, K
Costa, LG
NEUROTOXICOLOGY, 1996, 17 (02) : 523 - 530
[4] Differential effects of the M(1)-M(5) muscarinic acetylcholine receptor subtypes on intracellular calcium and on the incorporation of choline into membrane lipids in genetically modified Chinese hamster ovary cell lines
Dolezal, V
Lisa, V
Tucek, S
BRAIN RESEARCH BULLETIN, 1997, 42 (01) : 71 - 78
[5] TEMPERATURE EFFECTS ON THE ACTIVITY OF NICOTINIC ACETYLCHOLINE-RECEPTOR HETEROLOGOUSLY EXPRESSED IN CLONAL CELL-LINES
ZANELLO, LP
ANTOLLINI, S
ROCCAMO, AM
DEROMANELLI, IB
BARRANTES, FJ
BIOPHYSICAL JOURNAL, 1994, 66 (02) : A257 - A257
[6] Differential effects of traumatic brain injury on vesicular acetylcholine transporter and M2 muscarinic receptor mRNA and protein in rat
Shao, LF
Ciallella, JR
Yan, HQ
Ma, XC
Wolfson, BM
Marion, DW
Dekosky, ST
Dixon, CE
JOURNAL OF NEUROTRAUMA, 1999, 16 (07) : 555 - 566
[7] M3 Muscarinic Acetylcholine Receptor Expression Confers Differential Cholinergic Modulation to Neurochemically Distinct Hippocampal Basket Cell Subtypes
Cea-del Rio, Christian A.
Lawrence, J. Josh
Tricoire, Ludovic
Erdelyi, Ferenc
Szabo, Gabor
McBain, Chris J.
JOURNAL OF NEUROSCIENCE, 2010, 30 (17): : 6011 - 6024
[8] Preventive effects of bifemelane hydrochloride on decreased levels of muscarinic acetylcholine receptor and its mRNA in a rat model of chronic cerebral hypoperfusion
Kondo, Y
Ogawa, N
Asanuma, M
Matsuura, K
Nishibayashi, S
Iwata, E
NEUROSCIENCE RESEARCH, 1996, 24 (04) : 409 - 414
[9] MOLECULAR ANALYSIS OF BETA-ADRENERGIC-RECEPTOR SUBTYPES IN RAT COLLECTING DUCT - EFFECTS ON CELL CAMP AND CA2+ LEVELS
MANDON, B
SIGA, E
CHAMPIGNEULLE, A
IMBERTTEBOUL, M
ELALOUF, JM
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 1995, 268 (06) : F1070 - F1080
[10] Interaction of the nicotinic agonist (R,S)-3-pyridyl-1-methyl-2-(3-pyridyl)-azetidine (MPA) with nicotinic acetylcholine receptor subtypes expressed in cell lines and rat cortex
Zhang, X
Gong, ZH
Fasth, KJ
Långström, B
Nordberg, A
NEUROCHEMISTRY INTERNATIONAL, 1998, 32 (5-6) : 435 - 441

← 1 2 →