Production of D-lactate from glucose using Klebsiella pneumoniae mutants

被引:10
|
作者
Feng, Xinjun [1 ,2 ]
Jiang, Liqun [2 ]
Han, Xiaojuan [1 ]
Liu, Xiutao [1 ,3 ]
Zhao, Zhiqiang [1 ,3 ]
Liu, Huizhou [1 ]
Xian, Mo [1 ]
Zhao, Guang [1 ]
机构
[1] Chinese Acad Sci, CAS Key Lab Biobased Mat, Qingdao Inst Bioenergy & Bioproc Technol, Qingdao 266101, Peoples R China
[2] Chinese Acad Sci, Guangzhou Inst Energy Convers, CAS Key Lab Renewable Energy, Guangzhou 510640, Guangdong, Peoples R China
[3] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
基金
中国国家自然科学基金;
关键词
D-Lactate; Acetolactate synthase; Acetate kinase; Alcohol dehydrogenase; Klebsiella pneumoniae; D-LACTIC ACID; PURE D-LACTATE; ESCHERICHIA-COLI; SIMULTANEOUS SACCHARIFICATION; 3-HYDROXYPROPIONIC ACID; DEFICIENT MUTANT; 1,3-PROPANEDIOL; GLYCEROL; FERMENTATION; DEHYDROGENASE;
D O I
10.1186/s12934-017-0822-6
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Background: D-Lactate is a valued chemical which can be produced by some bacteria including Klebsiella pneumoniae. However, only a few studies have focused on K. pneumoniae for D-lactate production with a significant amount of by-products, which complicated the purification process and decreased the yield of D-lactate. Results: Based on the redirection of carbon towards by-product formation, the effects of single-gene and multiple-gene deletions in K. pneumoniae on D-lactate production from glucose via acetolactate synthase (budB), acetate kinase (ackA), and alcohol dehydrogenase (adhE) were tested. Klebsiella pneumoniae mutants had different production behaviours. The accumulation of the main by-products was decreased in the mutants. The triple mutant strain had the most powerful ability to produce optically pure D-lactate from glucose, and was tested with xylose and arabinose as carbon sources. Fed-batch fermentation was also carried out under various aeration rates, and the strain accumulated 125.1 g/L D-lactate with a yield of 0.91 g/g glucose at 2.5 vvm. Conclusions: Knocking out by-product synthesis genes had a remarkable influence on the production and yield of D-lactate. This study demonstrated, for the first time, that K. pneumoniae has great potential to convert monosaccharides into D-lactate. The results provide new insights for industrial production of D-lactate by K. pneumoniae.
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页数:11
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