A novel antifungal protein from seeds of Sesbania virgata (Cav.) Pers. (Leguminosae-Faboideae)

被引:6
|
作者
Praxedes, P. G. [1 ]
Zerlin, J. K. [1 ]
Dias, L. O. [1 ]
Pessoni, R. A. B. [1 ]
机构
[1] Univ Metodista Sao Paulo, Fac Saude, Curso Ciencias Biol, BR-09895400 Sao Bernardo Do Campo, SP, Brazil
基金
巴西圣保罗研究基金会;
关键词
plant defence; antimicrobial protein; filamentous fungi; seed protein; ANTIMICROBIAL PEPTIDES; CHITINASE; PLANTS; BEANS; CELLS;
D O I
10.1590/S1519-69842011000400013
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
A novel antifungal protein with a molecular mass around 50 kDa was purified from seeds of Sesbania virgata (Cav.) Pers. using ammonium sulfate fractionation followed by gel filtration on a Sephadex G-75 Superfine (Sigma) column and reverse-phase high performance liquid chromatography on a C8 column. The protein, designated FP1-A, with a novel N-terminal sequence AMVHSPGG(S) FS(P), showed growth inhibitory activity of filamentous fungi Aspergillus niger, Cladosporium cladosporioides, Colletotrichum gloeosporioides and Fusarium solani.
引用
收藏
页码:687 / 692
页数:6
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