Site-specific recombination in Arabidopsis plants promoted by the Integrase protein of coliphage HK022

被引：18
|
作者：
Gottfried, P
Lotan, O
Kolot, M
Maslenin, L
Bendov, R
Gorovits, R
Yesodi, V
Yagil, E [1 ]
Rosner, A
机构：
[1] Tel Aviv Univ, Dept Biochem, IL-69978 Tel Aviv, Israel
[2] FertiSeeds Ltd, IL-70400 Ness Ziona, Israel
[3] Agr Res Org, Volcani Ctr, Dept Virol, IL-50250 Bet Dagan, Israel
[4] Hebrew Univ Jerusalem, Fac Agr, Dept Plant Pathol & Microbiol, IL-76100 Rehovot, Israel
来源：
PLANT MOLECULAR BIOLOGY | 2005年 / 57卷 / 03期
关键词：
Arabidopsis; Integrase; nuclear localization; phage HK022; site-specific recombination;
D O I：
10.1007/s11103-004-0076-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The gene encoding the wild type Integrase protein of coliphage HK022 was integrated chromosomally and expressed in Arabidopsis thaliana plants. Double-transgenic plants cloned with the int gene as well as with a T-DNA fragment carrying the proper att sites in a tandem orientation showed that Int catalyzed a site-specific integration reaction (attP x attB) as well as a site-specific excision reaction (attL x attR). The reactions took place without the need to provide any of the accessory proteins that are required by Int in the bacterial host. When expressed in tobacco plants a GFP-Int fusion exhibits a predominant nuclear localization.
引用
收藏
页码：435 / 444
页数：10
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