Molecular cloning and characterization of a calreticulin cDNA from the pinewood nematode Bursaphelenchus xylophilus

被引：33

作者：

Li, Xundong ^{[1
,2
]}

Zhuo, Kan ^{[1
]}

Luo, Mei ^{[1
,3
]}

Sun, Longhua ^{[4
]}

Liao, Jinling ^{[1
]}

机构：

[1] S China Agr Univ, Lab Plant Nematol, Guangzhou 510642, Guangdong, Peoples R China

[2] Yunnan Acad Agr Sci, Yunnan 650205, Peoples R China

[3] Zhongkai Univ Agr & Engn, Guangzhou 510225, Guangdong, Peoples R China

[4] Natl Univ Singapore, Temasek Life Sci Lab, Biomat & Biocatalysts Grp, Singapore 117604, Singapore

来源：

EXPERIMENTAL PARASITOLOGY | 2011年 / 128卷 / 02期

关键词：

cDNA cloning; Bursaphelenchus xylophilus; Calreticulin; RACE; RNAi; RT-PCR; Southern blot; ROOT-KNOT NEMATODE; WOOD NEMATODE; CAENORHABDITIS-ELEGANS; IDENTIFICATION; FAMILY;

D O I：

10.1016/j.exppara.2011.02.017

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

The cloning and characterization of a cDNA encoding a calreticulin from the pinewood nematode Bursaphelenchus xylophilus is described herein. The full-length cDNA (Bx-crt-1) contained a 1200 bp open reading frame that could be translated to a 399 amino acid polypeptide. The deduced protein contained highly conserved regions of a calreticulin gene and had 66.2-70.1% amino acid sequence identity to other calreticulin sequences from nematodes. RNAi, RT-PCR amplification, and southern blot suggest that Bx-crt-1 may be important for the development of B. xylophilus. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：121 / 126

页数：6

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