A nonribosomal peptide synthetase gene tzw1 is involved in zwittermicin a biosynthesis in Bacillus thuringiensis G03

被引：6

作者：

Shao, Tiemei ^{[2
]}

Bai, Linquan ^{[3
,4
]}

Zhang, Jie ^{[2
]}

Wang, Guangjun ^{[2
]}

Liu, Daqun ^{[5
]}

Li, Zhuofu ^{[2
]}

Liu, Jianmi ^{[2
]}

Song, Fuping ^{[2
]}

Huang, Dafang ^{[1
]}

机构：

[1] Chinese Acad Agr Sci, Biotechnol Res Inst, Beijing 100081, Peoples R China

[2] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100094, Peoples R China

[3] Shanghai Jiao Tong Univ, Lab Microbial Metab, Shanghai 200030, Peoples R China

[4] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai 200030, Peoples R China

[5] Agr Univ Hebei, Coll Plant Protect, Baoding 071001, Peoples R China

来源：

CURRENT MICROBIOLOGY | 2008年 / 57卷 / 01期

关键词：

D O I：

10.1007/s00284-008-9153-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A 4.20-kb SspI fragment from Bacillus thuringiensis G03 was cloned and sequenced. Sequencing analysis revealed two complete open reading frames (ORF; tzw1 and tzw2), and one incomplete ORF (tzw3) (GenBank accession no. EU293887). Tzw1 encodes a putative nonribosomal peptide synthetase with thiolation and condensation domains localized at the C-termini, whereas tzw2 and tzw3 encode acyl carrier protein and Acyl-CoA dehydrogenase, respectively. To investigate the function of tzw1 in zwittermicin A (ZA) biosynthesis, an in-frame deletion of 1,461 bp within tzw1 was constructed. The mutant abolished ZA production. Complementation of the mutant with cloned tzw1 restored ZA productivity. These results revealed that tzw1 is required for ZA biosynthesis in B. thuringiensis G03.

引用

页码：61 / 65

页数：5

共 5 条

[1] A Nonribosomal Peptide Synthetase Gene tzw1 Is Involved in Zwittermicin A Biosynthesis in Bacillus thuringiensis G03
Tiemei Shao
Linquan Bai
Jie Zhang
Guangjun Wang
Daqun Liu
Zhuofu Li
Jianmi Liu
Fuping Song
Dafang Huang
[J]. Current Microbiology, 2008, 57
[2] The peptide synthetase gene phsA from Streptomyces viridochromogenes is not juxtaposed with other genes involved in nonribosomal biosynthesis of peptides
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[3] A nonribosomal peptide synthetase gene (mgoA) of Pseudomonas syringae pv. syringae is involved in mangotoxin biosynthesis and is required for full virulence
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[4] Use of a nonhomologous end joining deficient strain (Δku70) of the ergot fungus Claviceps purpurea for identification of a nonribosomal peptide synthetase gene involved in ergotamine biosynthesis
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[5] Genomic Island TnSmu2 of Streptococcus mutans Harbors a Nonribosomal Peptide Synthetase-Polyketide Synthase Gene Cluster Responsible for the Biosynthesis of Pigments Involved in Oxygen and H2O2 Tolerance
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