Effect of hepatic stimulator substance administration on tissue regeneration due to thioacetamide-induced liver injury in rats

被引:0
|
作者
Theocharis, SE
Margeli, AP
Agapitos, EV
Mykoniatis, MG
Kittas, CN
Davaris, PS
机构
[1] Univ Athens, Sch Med, Dept Pathol, GR-11527 Athens, Greece
[2] Univ Athens, Sch Med, Dept Expt Pharmacol, GR-11527 Athens, Greece
[3] Univ Athens, Sch Med, Dept Histol & Embryol, GR-11527 Athens, Greece
关键词
hepatic stimulator substance; injury; liver; regeneration; thioacetamide;
D O I
暂无
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Background: Hepatic stimulator substance (HSS) is a known hepatic growth factor that appears to be organ-specific but species-nonspecific, In the present study we investigated the effect of HSS administration in a rat model of liver injury and regeneration induced by thioacetamide (TAA) injection. Methods: TAA (300 mg/kg body weight) was injected intraperitoneally in male Wistar rats (group T). HSS (50 mg protein/kg body weight) was administered intraperitoneally either at 24 h (group II) or at 36 h (group IF) after TAA treatment. The animals were killed at different time points after TAA injection. and the rate of tritiated thymidine incorporation into hepatic DNA, the activity of the enzyme thymidine kinase in liver, and the assessment of the mitotic index in hepatocytes were used to estimate liver regeneration. Results: The administration of TAA caused severe hepatic injury recognized histopathologically and by increased activities of the serum hepatic enzymes aspartate and alanine aminotrasferases. The hepatic injury, which peaked at 24 h and 36 h after TAA injection, was followed by a regenerative process of hepatocytes which presented peaks after 48 h and 60 h (group I). The regenerative process of hepatocytes remained unaffected when HSS was administered 24 h after the injection of TAA (group II). In the case of HSS administration 36 h after the injection of TAA (group m) the examined indices of hepatocyte proliferation were statistically significantly increased at 48 h (P < 0.001), compared with those observed in group I. Conclusions: The administration of HSS enhanced the hepatocyte proliferative capacity, induced by TAA treatment, depending on the time of its administration.
引用
收藏
页码:656 / 663
页数:8
相关论文
共 50 条
  • [1] Effect of hepatic stimulator substance administration on thioacetamide-induced liver regeneration in rats.
    Theocharis, SE
    Agapitos, E
    Margeli, AP
    Mykoniatis, MG
    Davaris, PS
    [J]. GASTROENTEROLOGY, 1997, 112 (04) : A1401 - A1401
  • [2] Effect of granulocyte colony-stimulating-factor administration on tissue regeneration due to thioacetamide-induced liver injury in rats
    Theocharis, SE
    Margeli, AP
    Kittas, CN
    [J]. DIGESTIVE DISEASES AND SCIENCES, 1999, 44 (10) : 1990 - 1996
  • [3] Effect of Granulocyte Colony-Stimulating-Factor Administration on Tissue Regeneration Due to Thioacetamide-Induced Liver Injury in Rats
    Stamatios E. Theocharis
    Alexandra P. Margeli
    Christos N. Kittas
    [J]. Digestive Diseases and Sciences, 1999, 44 : 1990 - 1996
  • [4] Hepatic effect of sofosbuvir and daclatasvir in thioacetamide-induced liver injury in rats
    Ibrahim, Mohamed A.
    Abdel-Aziz, Asmaa
    El-Sheikh, Azza
    Kamel, Maha
    Khalil, Al-Zahraa
    Abdelhaleem, Hisham
    [J]. CLINICAL AND EXPERIMENTAL HEPATOLOGY, 2018, 4 (03) : 175 - 181
  • [5] Hepatic stimulator substance activity during thioacetamide induced liver injury in rats.
    Margeli, AP
    Skaltsas, S
    Mykoniatis, MG
    Theocharis, SE
    [J]. HEPATOLOGY, 1998, 28 (04) : 603A - 603A
  • [6] Hepatic stimulator substance protects rats against thioacetamide-induced fulminant hepatic failure and encephalopathy
    Theocharis, SE
    Papadimitriou, L
    Margeli, AP
    [J]. JOURNAL OF HEPATOLOGY, 2001, 34 : 45 - 45
  • [7] Effect of granulocyte-colony stimulating factor administration on thioacetamide-induced liver regeneration in rats.
    Theocharis, SE
    Margeli, AP
    Kittas, CN
    [J]. HEPATOLOGY, 1997, 26 (04) : 989 - 989
  • [8] Effect of dichloromethylene diphosphonate on liver regeneration following thioacetamide-induced necrosis in rats
    Bautista, Mirandeli
    Gomez del Rio, Maria Angeles
    Benedi, Juana
    Isabel Sanchez-Reus, Maria
    Morales-Gonzalez, Jose A.
    Maria Tellez-Lopez, Ana
    Lopez-Orozco, Maricela
    [J]. WORLD JOURNAL OF HEPATOLOGY, 2013, 5 (07) : 379 - 386
  • [9] Hepatic stimulator substance activity in the liver of thioacetamide-intoxicated rats
    Margeli, AP
    Skaltsas, SD
    Spiliopoulou, CA
    Mykoniatis, MG
    Theocharis, SE
    [J]. LIVER, 1999, 19 (06): : 519 - 525
  • [10] Effect of dichloromethylene diphosphonate on liver regeneration following thioacetamide-induced necrosis in rats
    Mirandeli Bautista
    María ngeles Gómez del Rio
    Juana Benedí
    María Isabel Sánchez-Reus
    José A Morales-González
    Ana María Téllez-López
    Maricela López-Orozco
    [J]. World Journal of Hepatology, 2013, (07) : 379 - 386