Generation of Functional Cardiomyocytes from Human Gastric Fibroblast-Derived Induced Pluripotent Stem Cells

被引:0
|
作者
Wu, Chih-Hsien [1 ,2 ]
Lin, Hsuan-Hwai [3 ]
Wu, Yi-Ying [4 ]
Chiu, Yi-Lin [1 ]
Huang, Li-Yen [2 ,5 ]
Cheng, Cheng-Chung [2 ]
Yang, Chung-Chi [2 ,5 ]
Tsai, Tsung-Neng [2 ]
机构
[1] Natl Def Med Ctr, Dept Biochem, Taipei 11490, Taiwan
[2] Tri Serv Gen Hosp, Div Cardiol, Dept Internal Med, Natl Def Med Ctr, Taipei 11490, Taiwan
[3] Tri Serv Gen Hosp, Div Gastroenterol, Dept Internal Med, Natl Def Med Ctr, Taipei 11490, Taiwan
[4] Tri Serv Gen Hosp, Div Hematol Oncol, Dept Med, Natl Def Med Ctr, Taipei 11490, Taiwan
[5] Taoyuan Armed Forces Gen Hosp, Div Cardiol, Dept Internal Med, Longtan Township 32551, Taoyuan County, Taiwan
关键词
induced pluripotent stem cells; human gastric fibroblast cells; cardiomyocytes; EMERGENCY ENDOSCOPY; EXPRESSION; INDUCTION;
D O I
10.3390/biomedicines9111565
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Coronary artery diseases are major problems of the world. Coronary artery disease patients frequently suffer from peptic ulcers when they receive aspirin treatment. For diagnostic and therapeutic purposes, the implementation of panendoscopy (PES) with biopsy is necessary. Some biopsy samples are wasted after the assay is completed. In the present study, we established a protocol for human gastric fibroblast isolation and induced pluripotent stem cell (iPSC) generation from gastric fibroblasts via PES with biopsy. We showed that these iPSCs can be differentiated into functional cardiomyocytes in vitro. To our knowledge, this is the first study to generate iPSCs from gastric fibroblasts in vitro.
引用
收藏
页数:13
相关论文
共 50 条
  • [1] Early selection of human fibroblast-derived induced pluripotent stem cells
    Stoyanova, Elena
    Mourdjeva, Milena
    Kyurkchiev, Stanimir
    [J]. BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT, 2015, 29 (05) : 942 - 948
  • [2] Functional Cardiomyocytes Derived From Human Induced Pluripotent Stem Cells
    Zhang, Jianhua
    Wilson, Gisela F.
    Soerens, Andrew G.
    Koonce, Chad H.
    Yu, Junying
    Palecek, Sean P.
    Thomson, James A.
    Kamp, Timothy J.
    [J]. CIRCULATION RESEARCH, 2009, 104 (04) : E30 - E41
  • [3] Induction of oligodendrocyte differentiation from adult human fibroblast-derived induced pluripotent stem cells
    Ogawa, Shin-ichiro
    Tokumoto, Yasuhito
    Miyake, Jun
    Nagamune, Teruyuki
    [J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 2011, 47 (07) : 464 - 469
  • [4] Induction of oligodendrocyte differentiation from adult human fibroblast-derived induced pluripotent stem cells
    Shin-ichiro Ogawa
    Yasuhito Tokumoto
    Jun Miyake
    Teruyuki Nagamune
    [J]. In Vitro Cellular & Developmental Biology - Animal, 2011, 47 : 464 - 469
  • [5] Generation and characterization of functional cardiomyocytes using induced pluripotent stem cells derived from human fibroblasts
    Gai, Hui
    Leung, Elaine Lai-Han
    Costantino, Peter D.
    Aguila, Jerell R.
    Nguyen, David M.
    Fink, Louis M.
    Ward, David C.
    Ma, Yupo
    [J]. CELL BIOLOGY INTERNATIONAL, 2009, 33 (11) : 1184 - 1193
  • [6] Generation and Characterization of Functional Cardiomyocytes Derived from Human T Cell-Derived Induced Pluripotent Stem Cells
    Seki, Tomohisa
    Yuasa, Shinsuke
    Kusumoto, Dai
    Kunitomi, Akira
    Saito, Yuki
    Tohyama, Shugo
    Yae, Kojiro
    Kishino, Yoshikazu
    Okada, Marina
    Hashimoto, Hisayuki
    Takei, Makoto
    Egashira, Toru
    Kodaira, Masaki
    Kuroda, Yusuke
    Tanaka, Atsushi
    Okata, Shinichiro
    Suzuki, Tomoyuki
    Murata, Mitsushige
    Fujita, Jun
    Fukuda, Keiichi
    [J]. PLOS ONE, 2014, 9 (01):
  • [7] Generating a Human Gonadal Cells Model from Terminal Differentiated Fibroblast-Derived Induced Pluripotent Stem Cells
    Gutierrez, Daniel Rodriguez
    Eid, Wassim
    Biason-Lauber, Anna
    [J]. HORMONE RESEARCH IN PAEDIATRICS, 2018, 90 : 20 - 20
  • [8] Establishment of a pancreatic stem cell line from fibroblast-derived induced pluripotent stem cells
    Kuise, Takashi
    Noguchi, Hirofumi
    Tazawa, Hiroshi
    Kawai, Takashi
    Iwamuro, Masaya
    Saitoh, Issei
    Kataoka, Hitomi Usui
    Watanabe, Masami
    Noguchi, Yasufumi
    Fujiwara, Toshiyoshi
    [J]. BIOMEDICAL ENGINEERING ONLINE, 2014, 13
  • [9] Establishment of a pancreatic stem cell line from fibroblast-derived induced pluripotent stem cells
    Takashi Kuise
    Hirofumi Noguchi
    Hiroshi Tazawa
    Takashi Kawai
    Masaya Iwamuro
    Issei Saitoh
    Hitomi Usui Kataoka
    Masami Watanabe
    Yasufumi Noguchi
    Toshiyoshi Fujiwara
    [J]. BioMedical Engineering OnLine, 13
  • [10] Generation of functional cardiomyocytes from mouse induced pluripotent stem cells
    So, Kyoung Ha
    Han, Young Joon
    Park, Hyo Young
    Kim, Jae Gon
    Sung, Dong Jun
    Bae, Young Min
    Yang, Byoung Chul
    Park, Soo Bong
    Chang, Sang Keun
    Kim, Eun Young
    Park, Se Pill
    [J]. INTERNATIONAL JOURNAL OF CARDIOLOGY, 2011, 153 (03) : 277 - 285