Generation of Functional Cardiomyocytes from Human Gastric Fibroblast-Derived Induced Pluripotent Stem Cells

被引：0
|
作者：
Wu, Chih-Hsien [1 ,2 ]
Lin, Hsuan-Hwai [3 ]
Wu, Yi-Ying [4 ]
Chiu, Yi-Lin [1 ]
Huang, Li-Yen [2 ,5 ]
Cheng, Cheng-Chung [2 ]
Yang, Chung-Chi [2 ,5 ]
Tsai, Tsung-Neng [2 ]
机构：
[1] Natl Def Med Ctr, Dept Biochem, Taipei 11490, Taiwan
[2] Tri Serv Gen Hosp, Div Cardiol, Dept Internal Med, Natl Def Med Ctr, Taipei 11490, Taiwan
[3] Tri Serv Gen Hosp, Div Gastroenterol, Dept Internal Med, Natl Def Med Ctr, Taipei 11490, Taiwan
[4] Tri Serv Gen Hosp, Div Hematol Oncol, Dept Med, Natl Def Med Ctr, Taipei 11490, Taiwan
[5] Taoyuan Armed Forces Gen Hosp, Div Cardiol, Dept Internal Med, Longtan Township 32551, Taoyuan County, Taiwan
来源：
BIOMEDICINES | 2021年 / 9卷 / 11期
关键词：
induced pluripotent stem cells; human gastric fibroblast cells; cardiomyocytes; EMERGENCY ENDOSCOPY; EXPRESSION; INDUCTION;
D O I：
10.3390/biomedicines9111565
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Coronary artery diseases are major problems of the world. Coronary artery disease patients frequently suffer from peptic ulcers when they receive aspirin treatment. For diagnostic and therapeutic purposes, the implementation of panendoscopy (PES) with biopsy is necessary. Some biopsy samples are wasted after the assay is completed. In the present study, we established a protocol for human gastric fibroblast isolation and induced pluripotent stem cell (iPSC) generation from gastric fibroblasts via PES with biopsy. We showed that these iPSCs can be differentiated into functional cardiomyocytes in vitro. To our knowledge, this is the first study to generate iPSCs from gastric fibroblasts in vitro.
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页数：13
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