Poly(3-hydroxybutyrate) production from xylose by recombinant Escherichia coli

被引:0
|
作者
Lee, SY
机构
[1] Korea Adv Inst Sci & Technol, Dept Chem Engn, Taejon 305701, South Korea
[2] Korea Adv Inst Sci & Technol, Bioproc Engn Res Ctr, Taejon 305701, South Korea
来源
BIOPROCESS ENGINEERING | 1998年 / 18卷 / 05期
关键词
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Several recombinant Escherichia coli strains harboring the Alcaligenes eutrophus polyhydroxyalkanoate biosynthesis genes were used to produce poly(3-hydroxybutyrate), PHB, from xylose. By flask culture of TG1 (pSYL107) in a defined medium containing 20 g/l xylose, PHB concentration of 1.7 g/l was obtained. Supplementation of a small amount of cotton seed hydrolysate or soybean hydrolysate could enhance PHB production by more than two fold. The PHB concentration, PHB content, and PHB yield on xylose obtained by supplementing soybean hydrolysate were 4.4 g/l, 73.9%, and 0.226 g PHB/g xylose, respectively.
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页码:397 / 399
页数:3
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