A SIMPLE METHOD FOR CRYOPRESERVATION OF SHOOT TIPS OF Arabidopsis GENOTYPES

被引：0
|
作者：
Stock, Johanna [1 ]
Senula, Angelika [1 ]
Nagel, Manuela [1 ]
Mock, Hans-Peter [1 ]
Keller, E. R. Joachim [1 ]
机构：
[1] Leibniz Inst Plant Genet & Crop Plant Res IPK, Corrensstr 3, D-06466 Seeland Ot Gatersleben, Germany
来源：
CRYOLETTERS | 2017年 / 38卷 / 05期
关键词：
Arabidopsis thaliana; cryopreservation; DSC; PVS2; shoot tips; DROPLET-VITRIFICATION; COLD-ACCLIMATION; PLANT; THALIANA; CELLS; DEHYDRATION; GERMPLASM; PROTOCOL; STRESS; POTATO;
D O I：
暂无
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
BACKGROUND: The use of the model plant Arabidopsis could be a valuable tool to elucidate the basic mechanisms involved in plant cryopreservation. OBJECTIVE: A simple and powerful protocol, independent of Arabidopsis genotypes, was established using a PVS2 protocol. MATERIALS AND METHODS: Two PVS2 (a and b), one PVS3 droplet-vitrification and one DMSO droplet-freezing protocol were tested with alternating temperatures during the growing phase of donor plants. RESULTS: PVS2 protocols, including cold acclimation of donor plants, resulted in highest recovery. The PVS2a protocol was successfully applied to a collection of different Arabidopsis genotypes with an average recovery of 94%. In addition, Differential Scanning Calorimetry confirmed the occurrence of glass transitions in the PVS3 and PVS2 protocols. CONCLUSION: The PVS2a protocol is suitable to screen the large collection of Arabidopsis mutants and transgenic lines with the aim to identify cellular functions associated with cryopreservation tolerance.
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页码：364 / 371
页数：8
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