Immunological detection of proteolytically activated epidermal-type transglutaminase (TGase 3) using cleavage-site-specific antibody

被引：8
|
作者：
Hitomi, K [1 ]
Ikeda, N [1 ]
Maki, M [1 ]
机构：
[1] Nagoya Univ, Grad Sch Bioagr Sci, Dept Appl Mol Biol Sci, Nagoya, Aichi 4648601, Japan
来源：
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2003年 / 67卷 / 11期
关键词：
transglutaminase; proteolysis; forestomach; keratinocyte;
D O I：
10.1271/bbb.67.2492
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Transglutaminase 3 (TGase 3), involved in the cross-linking of structural proteins in the epidermis, is activated by limited proteolysis of zymogen into two fragments during keratinocyte differentiation. Using recombinant TGase 3, the N-terminus sequence of the proteolyzed fragment was analyzed. Antibody against the synthetic peptide corresponding to the cleavage site specifically detected the fragment in the mouse forestomach extract.
引用
收藏
页码：2492 / 2494
页数：3
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