Evaluating the radioprotective effect of hesperidin in the liver of Swiss albino mice

The present study was aimed to evaluate the radioprotective efficacy of hesperidin, a flavonone glycoside against X-ray radiation-induced cellular damage in the liver of Swiss albino mice. The first phase of the study was carried out to fix the effective concentration of hesperidin by performing a 30 days of survival studies using different graded doses [12.5, 25, 50 and 100 mg/kg body weight] of hesperidin administered orally to mice via intragastric intubations for seven consecutive days prior to exposure of whole body radiation (10 Gy). Based on the results of survival studies, the effective dose of hesperidin was fixed which was then administered to animals orally via intragastric intubations for seven consecutive days prior to exposure of whole body radiation (4 Gy) to evaluate its radioprotective efficacy by performing various biochemical estimations, comet assay, DNA fragmentation assay and histopathological studies in the liver of Swiss albino mice. The results indicated that radiation-induced decrease in the levels of endogenous antioxidant enzymes and increase in lipid peroxidative index, DNA damage and comet parameters were altered by pre-administration with the effective dose of hesperidin [25 mg/kg body weight] which restored the antioxidant status to near normal and decreased the levels of lipid peroxidative index. DNA damage and comet parameters. These results were further confirmed by histopathological examinations which indicated that pre-administration with the effective dose of hesperidin reduced the hepatic damage induced by radiation. Thus the current study shows hesperidin to be an effective radioprotector against radiation induced damage in the liver of mice. (C) 2011 Elsevier B.V. All rights reserved.