Comparison of the determination of affinity constants with surface plasmon resonance and quartz crystal microbalance

被引：0
|
作者：
Kösslinger, C [1 ]
Uttenthaler, E [1 ]
Abel, T [1 ]
Hauck, S [1 ]
Drost, S [1 ]
机构：
[1] Fraunhofer Inst Solid State Technol, Munich, Germany
来源：
EUROSENSORS XII, VOLS 1 AND 2 | 1998年
关键词：
D O I：
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中图分类号：
T [工业技术];
学科分类号：
08 ;
摘要：
A comparison between quartz crystal microbalance (QCM) and surface plasmon resonance (SPR) as devices for the determination of affinity constants is presented. The association and dissociation rate constants k(d) and k(a) respectively were determined by non-linear curve fitting of the time dependent measurement signals during association and dissociation processes. We used the virus-protein vp73 from the african swine fever (ASF) virus as analyte and immobilised an anti-ASF-antibody. The SPR measurements yielded 7.9.10(-4) s(-1) for k(d) and 5.6.10(3) (Ms)(-1) for k(a). The QCM results were 3.1.10(-4) s(-1) for k(d) and 1.1.10(4) (Ms)(-1) for k(a). k(d) and k(a) differ for about a factor of 2 for the QCM and SPR respectively.
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页码：845 / 848
页数：4
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