mRNA translation is not a prerequisite for small interfering RNA-mediated mRNA cleavage

被引：14
|
作者：
Sen, GL
Wehrman, TS
Blau, HM
机构：
[1] Stanford Univ, Sch Med, Baxter Lab Genet Pharmacol, Stanford, CA 94305 USA
[2] Stanford Univ, Sch Med, Dept Mol Pharmacol, Stanford, CA 94305 USA
[3] Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA
来源：
DIFFERENTIATION | 2005年 / 73卷 / 06期
关键词：
RNAi; siRNA; translation; RISC; gene regulation;
D O I：
10.1111/j.1432-0436.2005.00029.x
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
RNA interference constitutes a major means of eliminating mRNAs, yet how the small interfering RNAs (siRNA) within the RNA-induced silencing complex (RISC) finds its homologous target in the cell remains unknown. An attractive hypothesis is that RNA interference is linked to translation which allows RISC ready access to every translated mRNA. To test whether translation could direct siRNAs to mRNAs, chemical and biological inhibitors of translation and their effects on mRNA cleavage were tested. Our results show that mRNA degradation by siRNAs is not dependent on mRNA translation.
引用
收藏
页码：287 / 293
页数：7
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