Analysis of oxidative deoxyribonucleic acid damage marker, 8-hydroxy-2′-deoxyguanosine, by high performance liquid chromatography with electrochemical detection

被引：0

作者：

Xi, ZG ^{[1
]}

Chao, FH ^{[1
]}

Sun, YM ^{[1
]}

Yang, DF ^{[1
]}

Zhang, HS ^{[1
]}

机构：

[1] Acad Mil Med Sci, Inst Hlth & Environm Med, Tianjin 300050, Peoples R China

来源：

CHINESE JOURNAL OF ANALYTICAL CHEMISTRY | 2001年 / 29卷 / 07期

关键词：

high performance liquid chromatography; electrochemical detection; oxidative deoxyribonucleic acid damage; 8-hydroxy-2 '-deoxyguanosine;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Using chelator-Fe2+-H2O2 as the oxidative source of Fenton type reaction, reacting with calf thymus deoxyribonucleic acid (DNA) in vitro model. The yielded, 8-hydroxy-2'-deoxyguanosine (8-OhdG), was analyzed by high performance liquid chromatography with electrochemical detection. The method established has higher sensitivity, the detection limit is up to 30 fmol, wider linear range is from 0.32 pmol to 32nmol, and the correlation coefficient is 0.9996. Ibis method could he used to determine the low oxidative DNA damage in organism or cell level.

引用

页码：765 / 767

页数：3

共 4 条

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AMES, BN
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