Preparation and Application in Capturing Bacteria of Submicron Immunomagnetic Beads

被引：0
|
作者：
Niu Mu [1 ]
Du Mei-Hong [2 ]
Deng Yi [1 ,2 ]
Gao Ming-Yuan [1 ]
机构：
[1] Chinese Acad Sci, Inst Chem, Beijing 100190, Peoples R China
[2] Beijing Ctr Phys & Chem Anal, Beijing 100089, Peoples R China
来源：
CHEMICAL JOURNAL OF CHINESE UNIVERSITIES-CHINESE | 2011年 / 32卷 / 02期
关键词：
Submicron; Immunomagnetic beads; Salmonella; Magnetic capture efficiency; POLYMER MICROSPHERES; MAGNETIC MICROSPHERES; PARTICLES; SEPARATION; NANOPARTICLES; SALMONELLA; ALBUMIN; FE3O4; WATER;
D O I：
暂无
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Based on the submicron monodispersed magnetic beads, the immunomagnetic beads covered with anti-Salmonella antibody were obtained. The adsorption behavior of IgG on the surface of magnetic beads was investigated through surface electric potential, fluorescence and enzyme-linked immunosorbent assay (ELISA) method. In the immunoassay experiment, by adjusting the initial concentration of IgG, the relativity between surface concentration of IgG on magnetic beads and magnetic capture efficiency was also investigated. Finally, comparison with commercial Dynabeads, the submicron immunomagnetic beads represents higher capture efficiency for Salmonella.
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页码：322 / 326
页数：5
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