Differential dendritic immune cell responses to infection with various serotypes of Shigella

被引:0
|
作者
Narayan, Chandradeo [1 ]
Kant, Vishal [1 ]
Mahajan, Jai Kumar [1 ]
Mohan, Balvinder [1 ]
Taneja, Neelam [1 ]
机构
[1] Post Grad Inst Med Educ & Res, Dept Med Microbiol, Chandigarh, India
关键词
Shigella; Monocyte; Dendritic cell; Pro-inflammatory cytokines; ESCHERICHIA-COLI; SECRETION; RECOGNITION; DESTRUCTION; RESISTANCE; FLEXNERI; SONNEI;
D O I
10.1016/j.ijmmb.2022.02.007
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Purpose: Dendritic cells (DC) are key regulators of immune response with the ability to affect both the innate and adaptive immune responses and are abundant in the gut mucosa. The severity of shigellosis varies with the serotype involved with S. dysenteriae producing the severest infections with complications and S. sonnei being at the other end of spectrum usually causing mild self-limiting diarrhea. While Shigella are known to induce the apoptosis of mature DCs, there is no information on cytokine milieu of DCs incubated with different serotypes of Shigella. Methods: Monocyte derived dendritic cells (MoDCs) were developed from healthy human PBMC after 8 days of culture. DCs were infected with different Shigella serotypes. After 24 h post infection, relative expression of cytokines IL-1 beta, IL-6, IL-8, TNF-alpha, IL-12p70, IL-17, IL-22 and IL-23 was studied by Real Time PCR and cytometric bead arrays (CBA). Results: We found that different serotypes of Shigella significantly stimulated production of IL-1 beta, IL-6, IL-8, IL-12, IL-23, INF-gamma and TNF-alpha compared to uninfected DCs and there were significant differences among these serotypes. At transcriptional level, highest levels of expression of IL-1 beta, IL-6, IL-8, TNF-alpha, IFN-gamma, IL-17, IL-22 and IL-23 were observed in S. dysenteriae infected DCs. Significant serotypic differences were noted between S. dysenteriae & S. flexneri and between S. dysenteriae & S. sonnei. Conclusions: DCs are critical sentinel cells that relay microbial presence either directly or indirectly to naive T cells. In this study we found that S. dysenteriae caused maximum expression of pro-inflammatory cytokines. Similarly, S. dysenteriae also caused highest expression of IL-17A and IL-22A. It was the only serotype, which increased IL-23. These findings could explain more severity of SD as compared to SF and SS.
引用
收藏
页码:217 / 222
页数:6
相关论文
共 50 条
  • [1] Differential dendritic cells responses to infection with various serotypes of shigella
    Narayan, C.
    Thapa, B. R.
    Mahajan, J. K.
    Kant, V.
    Mohan, B.
    Taneja, N.
    [J]. INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES, 2016, 45 : 78 - 78
  • [2] Dendritic cell control of immune responses
    Morel, Penelope A.
    Butterfield, Lisa H.
    [J]. FRONTIERS IN IMMUNOLOGY, 2015, 6
  • [3] Dendritic cell dendritic cell interactions in primary immune responses.
    Knight, SC
    Bedford, PA
    Garner, K
    Kelleher, P
    [J]. JOURNAL OF LEUKOCYTE BIOLOGY, 1998, : 11 - 11
  • [4] Differential invasiveness & expression of antimicrobial peptides in Shigella serotypes
    Narayan, Chandradeo
    Kant, Vishal
    Mahajan, Jai Kumar
    Mohan, Balvinder
    Taneja, Neelam
    [J]. INDIAN JOURNAL OF MEDICAL RESEARCH, 2023, 158 (03) : 303 - +
  • [5] Dendritic cell subsets in mucosal immune responses
    Agace, W.
    [J]. IMMUNOLOGY, 2014, 143 : 21 - 21
  • [6] SUSCEPTIBILITY OF CELL CULTURES FROM VARIOUS MAMMALIAN TISSUES TO INFECTION BY SHIGELLA
    OGAWA, H
    YOSHIKURA, H
    NAKAMURA, A
    NAKAYA, R
    [J]. JAPANESE JOURNAL OF MEDICAL SCIENCE & BIOLOGY, 1967, 20 (04): : 329 - +
  • [7] RHEUMATOID DENDRITIC CELLS AS ACCESSORY CELLS FOR VARIOUS IMMUNE-RESPONSES
    FORRE, O
    WAALEN, K
    PAHLE, J
    [J]. IMMUNOBIOLOGY, 1984, 167 (1-3) : 117 - 117
  • [8] Role of osteopontin in dendritic cell shaping of immune responses
    Del Prete, Annalisa
    Scutera, Sara
    Sozzani, Silvano
    Musso, Tiziana
    [J]. CYTOKINE & GROWTH FACTOR REVIEWS, 2019, 50 : 19 - 28
  • [9] Myeloid dendritic cell lectins and their role in immune responses
    Dominguez-Soto, Angeles
    Corbi, Angel L.
    [J]. CURRENT OPINION IN INVESTIGATIONAL DRUGS, 2007, 8 (11) : 910 - 920
  • [10] Dendritic cell subsets in the regulation of intestinal immune responses
    Luda, K.
    Persson, E.
    Joeris, T.
    Lambrecht, B.
    Agace, W.
    [J]. IMMUNOLOGY, 2014, 143 : 6 - 6