Synthesis of 3′-thioamido-modified 3′-deoxythymidine-5′-triphosphates and their use as chain terminators in sanger-DNA sequencing

被引：7
|
作者：
Schwarzer, K [1 ]
Wojczewski, C [1 ]
Engels, JW [1 ]
机构：
[1] Univ Frankfurt, D-6000 Frankfurt, Germany
来源：
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS | 2001年 / 20卷 / 4-7期
关键词：
D O I：
10.1081/NCN-100002450
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The thioamide derivatives 3'-deoxy-5'-O-(4,4'-dimethoxytrityl)-3'-[(2-methyl-1-thioxo-propyl)amino]thymidine 1 and 3'-deoxy-5'-O-(4,4'-dimethoxytrityl) -3'- {{6-{[(9H-(fluo-ren-9-ylmethoxy)carbonyl] -amino}-1-thioxohexyl}amino} thymidine 2 were synthesized by regioselective thionation of their corresponding amides 7 and 8 with 2,4-bis(4-methoxyphenyl)-1,3,2,4-dithiadiphosphetane- 2,4-disulfide (Lawesson's reagent). The thioamides were converted into the corresponding 5'-triphosphates 3 and 4. Compound 3 was chosen for DNA sequencing experiments and 4 was further labelled with fluorescein.
引用
收藏
页码：879 / 882
页数：4
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