Identification of intimin alleles in pathogenic Escherichia coli by PCR-restriction fragment length polymorphism analysis

被引：11
|
作者：
Tramuta, C. [1 ]
Robino, P. [1 ]
Oswald, E. [2 ]
Nebbia, P. [1 ]
机构：
[1] Univ Turin, Dipartimento Prod Anim Epidemiol & Ecol, Fac Vet Med, I-10095 Grugliasco, TO, Italy
[2] Ecole Natl Vet Toulouse, INRA Microbiol Mol, UMR960, F-31000 Toulouse, France
来源：
VETERINARY RESEARCH COMMUNICATIONS | 2008年 / 32卷 / 01期
关键词：
Escherichia coli; intimin; PCR-RFLP;
D O I：
10.1007/s11259-007-9009-7
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
A rapid two-step identification method based on PCR-RFLP analysis of the intimin gene was developed to differentiate specific alleles in pathogenic Escherichia coli. This technique, tested on isolates eae-positive, accurately detects eae and resolves alleles encoding the alpha 1, alpha 2, beta, gamma 1, gamma 2/theta, kappa, epsilon, zeta, and iota intimin variants.
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页码：1 / 5
页数：5
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