Effective T-cell recall responses require the taurine transporter Taut

T-cell activation and the subsequent transformation of activated T cells into T-cell blasts require profound changes in cell volume. However, the impact of cell volume regulation for T-cell immunology has not been characterized. Here we studied the role of the cell-volume regulating osmolyte transporter Taut for T-cell activation in Taut-deficient mice. T-cell mediated recall responses were severely impaired in taut-/- mice as shown with B16 melanoma rejection and hapten-induced contact hypersensitivity. CD4+ and CD8+ T cells were unequivocally located within peripheral lymph nodes of unprimed taut-/- mice but significantly decreased in taut-/- compared with taut+/+ mice following in vivo activation. Further analysis revealed that Taut is critical for rescuing T cells from activation-induced cell death in vitro and in vivo as shown with TCR, superantigen, and antigen-specific activation. Consequently, reduction of CD4+ and CD8+ T cells in taut-/- mice upon antigen challenge resulted in impaired in vivo generation of T-cell memory. These findings disclose for the first time that volume regulation in T cells is an element in the regulation of adaptive immune responses and that the osmolyte transporter Taut is crucial for T-cell survival and T-cell mediated immune reactions.