Purpose: To evaluate the retention and marginal staining of restorations placed in non-carious cervical lesions using the all-in-one self-etching system, One-Up Bond F and Palfique Estelite resin composite. Methods: Fifty-one non-undercut non-carious cervical lesions received restorations with One-Up Bond F and Palfique Estelite in 15 subjects (mean age 57.7 years) by one operator. Teeth were cleaned with pumice and water, restored following the manufacturer's instructions and evaluated at 6 months, 1, 2, 4 and 5 years for retention and presence of marginal staining. Results: At 1 year, 42 restorations were evaluated, and all were intact with three restorations showing slight marginal staining. At 2 years, 37 restorations were evaluated from 11 subjects, and all were intact. Marginal staining was observed around nine restorations. At 4 years, 27 restorations and at 5 years, 24 restorations could be evaluated. Marginal staining showed a significant increase at the enamel and cervical margins over the 5 years of the study, with two restorations showing severe, nine mild and 13 no staining. Although a number of restorations could not be evaluated, the retention rate of 92% at 5 years showed One-Up Bond F/Palfique Estelite resin composite for the restoration of non-carious cervical lesions produced an excellent outcome. Marginal staining was limited to a small number of subjects and is unlikely to be a problem if margins are refurbished at recall visits.