Propofol Reversed Hypoxia-Induced Docetaxel Resistance in Prostate Cancer Cells by Preventing Epithelial-Mesenchymal Transition by Inhibiting Hypoxia-Inducible Factor 1α

被引:37
|
作者
Qian, Jiang [1 ]
Shen, Sheliang [2 ]
Chen, Wei [3 ]
Chen, Nianping [4 ]
机构
[1] Zhejiang Hosp, Dept Anesthesiol, Hangzhou 310014, Zhejiang, Peoples R China
[2] Zhejiang Prov Peoples Hosp, Dept Anesthesiol, Hangzhou 310013, Zhejiang, Peoples R China
[3] Univ Chicago, Inst Mol Engn, Chicago, IL 60637 USA
[4] Zhejiang Univ, Shaoxing Hosp, Shaoxing Peoples Hosp, Dept Anesthesiol, Shaoxing 312000, Zhejiang, Peoples R China
关键词
SUPPRESSES PROLIFERATION; CEREBRAL-ISCHEMIA; DOWN-REGULATION; STEM-CELLS; INVASION; HIF-1-ALPHA; APOPTOSIS; GROWTH; PLASTICITY; ERA;
D O I
10.1155/2018/4174232
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Prostate cancer is the second most frequently diagnosed cancer worldwide. Hypoxia-induced epithelial-mesenchymal transition (EMT), driven by hypoxia-inducible factor 1 alpha (HIF-1 alpha), is involved in cancer progression and metastasis. The present study was designed to explore the role of propofol in hypoxia-induced resistance of prostate cancer cells to docetaxel. We used the Cell Counting Kit-8 and 5-ethynyl-2' -deoxyuridine incorporation assay to measure cell viability and cell proliferation, respectively, in prostate cancer cell lines. Then, we detected HIF-1 alpha, E-cadherin, and vimentin expression using western blotting. Propofol reversed the hypoxia-induced docetaxel resistance in the prostate cancer cell lines. Propofol not only decreased hypoxia-induced HIF-1 alpha expression, but also reversed hypoxia-induced EMT by suppressing HIF-1 alpha. Furthermore, small interfering RNA-mediated silencing of HIF-1 alpha reversed the hypoxia-induced docetaxel resistance, although there was little change in docetaxel sensitivity between the hypoxia group and propofol group. The induction of hypoxia did not affect E-cadherin and vimentin expression, and under the siRNA knockdown conditions, the effects of propofol were obviated. These data support a role for propofol in regulating EMT in prostate cancer cells. Taken together, our findings demonstrate that propofol plays an important role in hypoxia-induced docetaxel sensitivity and EMT in prostate cancer cells and that it is a potential drug for overcoming drug resistance in prostate cancer cells via HIF-1 alpha suppression.
引用
收藏
页数:9
相关论文
共 50 条
  • [1] Hypoxia-induced alveolar epithelial-mesenchymal transition requires mitochondrial ROS and hypoxia-inducible factor 1
    Zhou, Guofei
    Dada, Laura A.
    Wu, Minghua
    Kelly, Aileen
    Trejo, Humberto
    Zhou, Qiyuan
    Varga, John
    Sznajder, Jacob I.
    [J]. AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 2009, 297 (06) : L1120 - L1130
  • [2] Hypoxia-inducible factor 1α participates in hypoxia-induced epithelial-mesenchymal transition via response gene to complement 32
    Zhu, Liang
    Zhao, Qiu
    [J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE, 2017, 14 (02) : 1825 - 1831
  • [3] Parthenolide suppresses hypoxia-inducible factor-1α signaling and hypoxia induced epithelial-mesenchymal transition in colorectal cancer
    Kim, Se Lim
    Park, Young Ran
    Lee, Soo Teik
    Kim, Sang-Wook
    [J]. INTERNATIONAL JOURNAL OF ONCOLOGY, 2017, 51 (06) : 1809 - 1820
  • [4] Hypoxia-inducible factor-1α induces the epithelial-mesenchymal transition of human prostatecancer cells
    Luo Yong
    He Da-lin
    Ning Liang
    Shen Shu-lin
    Li Lei
    Li Xiang
    [J]. CHINESE MEDICAL JOURNAL, 2006, 119 (09) : 713 - 718
  • [5] Hypoxia-inducible factor-1α induces the epithelial-mesenchymal transition of human prostatecancer cells
    LUO Yong HE Dalin NING Liang SHEN Shulin LI Lei and LI Xiang Department of Urology First Hospital of Xian Jiaotong University Xian China
    [J]. Chinese Medical Journal., 2006, (09) - 718
  • [6] Hypoxia-inducible factor 1α-induced epithelial-mesenchymal transition of endometrial epithelial cells may contribute to the development of endometriosis
    Xiong, Yao
    Liu, Yi
    Xiong, Wenqian
    Zhang, Ling
    Liu, Hengwei
    Du, Yu
    Li, Na
    [J]. HUMAN REPRODUCTION, 2016, 31 (06) : 1327 - 1338
  • [8] Not Only Hypoxia- but Radiation-Induced Epithelial-Mesenchymal Transition Is Modulated by Hypoxia-Inducible Factor 1 in A549 Lung Cancer Cells
    Sato, M.
    Hirose, K.
    Ichise, K.
    Yoshino, H.
    Harada, T.
    Hatayama, Y.
    Kawaguchi, H.
    Tanaka, M.
    Fujioka, I
    Takai, Y.
    Aoki, M.
    [J]. FOLIA BIOLOGICA, 2021, 67 (02) : 62 - 69
  • [9] Role of Wnt/β-catenin signaling pathway in epithelial-mesenchymal transition of human prostate cancer induced by hypoxia-inducible factor-1α
    Jiang, Yong-Guang
    Luo, Yong
    He, Da-lin
    Li, Xiang
    Zhang, Lin-lin
    Peng, Tao
    Li, Ming-Chuan
    Lin, Yun-Hua
    [J]. INTERNATIONAL JOURNAL OF UROLOGY, 2007, 14 (11) : 1034 - 1039
  • [10] Hypoxia-Inducible Factor 1 is required for hypoxia-induced resistance to doxorubicin in breast carcinoma cells
    Sullivan, Richard
    Frederiksen, Lisa J.
    Pare, Genevieve C.
    Graham, Charles H.
    [J]. CANCER RESEARCH, 2006, 66 (08)