Associations of physical activity, cardiorespiratory fitness and fatness with low-grade inflammation in adolescents: the AFINOS Study

Objective: To examine the independent associations of objectively measured physical activity (PA), cardiorespiratory fitness (CRF) and fatness with low-grade inflammatory markers in adolescents. Design: Cross-sectional study in Spain. Subjects: A sample of 192 adolescents aged 13-17 years. Measurements: PA was assessed with an accelerometer for 7 days. A 20-m shuttle-run test was used to assess CRF. Skinfold thicknesses at six sites and WCs were measured. BMI was calculated from measured height and weight. C-reactive protein (CRP), interleukin-6 (IL-6) and complement factors C3 and C4 were assayed. The homeostasis model assessment of insulin resistance (HOMA-IR) was calculated from glucose and insulin. Regression analysis adjusted for potential confounders and HOMA-IR was used to determine the associations between PA, CRF and fatness with low-grade inflammatory markers. Results: Total PA, vigorous PA and MVPA were positively associated with CRF (r=0.25-0.48), whereas vigorous PA was negatively associated with skinfolds (r=0.27). CRF was inversely associated with fatness, (r=0.30 to -0.48). CRF and fatness were inversely and positively associated with HOMA-IR (r=0.16 and 0.21, respectively). PA variables were not independently associated with inflammatory markers. CRF and fatness were inversely and positively associated with CRP, C3 and C4, respectively. Only body fat explained a relevant amount of the variance of the model in CRP (4%) and C4 (19%), whereas CRP and body fat jointly explained the variance in C3 (25%). All these observations were independent of HOMA-IR. Conclusions: These findings support the key role of CRF and fatness on low-grade inflammation, as well as the possible indirect role of habitual PA through CRF and body fat in adolescents. International Journal of Obesity (2010) 34, 1501-1507; doi:10.1038/ijo.2010.114; published online 8 June 2010