Analysis of cell-free fetal DNA in plasma and serum of pregnant women

被引：2

作者：

Zolotukhina, TV ^{[1
]}

Shilova, NV ^{[1
]}

Voskoboeva, EY ^{[1
]}

机构：

[1] Russian Acad Med Sci, Med Genet Res Ctr, Moscow 115478, Russia

来源：

JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY | 2005年 / 53卷 / 03期

关键词：

fetal DNA; maternal serum and plasma; SRY; nested PCR;

D O I：

10.1369/jhc.4B6398.2005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Sixty blood-samples from pregnant women during gestational weeks 9-28 were investigated. Cell-free fetal DNA was extracted from maternal plasma or serum to be detected by-nested PCR for determination of fetal gender. The SRY gene as a marker for fetal Y chromosome was detected-in 34/36 women carrying a male fetus. In 3/24 women carrying female fetuses, the SRY sequence was also detected. Overall, fetal sex was correctly predicted in 91.7% of-the cases. Therefore, the new, non-invasive method of prenatal diagnosis of fetal gender for women at risk of producing children with X-linked disorders is reliable, secure, and can substantially reduce invasive prenatal tests.

引用

页码：297 / 299

页数：3

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