Simultaneous detection of Salmonella spp, Salmonella Typhi and Salmonella Typhimurium in sliced fruits using multiplex PCR

被引：67
|
作者：
Pui, Chai Fung [1 ]
Wong, Woan Chwen [1 ]
Chai, Lay Ching [1 ]
Nillian, Elexson [1 ]
Ghazali, Farinazleen Mohamad [1 ]
Cheah, Yoke Kqueen [2 ]
Nakaguchi, Yoshitsugu [3 ]
Nishibuchi, Mitsuaki [3 ]
Radu, Son [1 ]
机构：
[1] Univ Putra Malaysia, Dept Food Sci, Fac Food Sci & Technol, Ctr Excellence Food Safety Res, Upm Serdang 43400, Selangor Darul, Malaysia
[2] Univ Putra Malaysia, Dept Biomed Sci, Fac Med & Hlth Sci, Upm Serdang 43400, Selangor Darul, Malaysia
[3] Kyoto Univ, Ctr SE Asian Studies, Kyoto 6068501, Japan
来源：
FOOD CONTROL | 2011年 / 22卷 / 02期
关键词：
SALAD VEGETABLES; FRESH PRODUCE; PREVALENCE; IDENTIFICATION; PATHOGENS; SPP; RAW; QUANTIFICATION; OUTBREAKS; CULTURE;
D O I：
10.1016/j.foodcont.2010.05.021
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
This study aimed to determine the prevalence and quantity of Salmonella spp Salmonella Typhi and Salmonella Typhimurium in sliced fruits from hawker stalls and hypermarkets in Malaysia Analysis was carried out using the most probable number (MPN) multiplex polymerase chain reaction (PCR) method The prevalence of Salmonella spp Salmonella Typhi and Salmonella Typhimurium in 210 samples of sliced fruits examined were 23 3% 7 6% and 3 8% respectively with estimated quantity varying from 0 to 19 MPN/g This study urged the authority to look into the biosafety of sliced fruits in Malaysia (C) 2010 Elsevier Ltd All rights reserved
引用
收藏
页码：337 / 342
页数：6
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