Bottlenecks in Proteomics: An Update

被引：2
|
作者：
Channaveerappa, Devika [1 ]
Wetie, Armand G. Ngounou [1 ]
Darie, Costel C. [1 ]
机构：
[1] Clarkson Univ, Dept Chem & Biomol Sci, Biochem & Prote Grp, Potsdam, NY 13699 USA
来源：
ADVANCEMENTS OF MASS SPECTROMETRY IN BIOMEDICAL RESEARCH, 2ND EDITION | 2019年 / 1140卷
关键词：
Mass spectrometry; Proteomics; Biochemical fractionation; SAMPLE PREPARATION METHOD; POLYACRYLAMIDE-GEL-ELECTROPHORESIS; PROTEIN-PROTEIN INTERACTIONS; MASS-SPECTROMETRY; DISULFIDE BOND; IDENTIFICATION; PRECIPITATION; TANDEM; GLYCOSYLATION; EXTRACTION;
D O I：
10.1007/978-3-030-15950-4_45
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Mass spectrometry (MS) is the core for advanced methods in proteomic experiments. When effectively used, proteomics may provide extensive information about proteins and their post-translational modifications, as well as their interaction partners. However, there are also many problems that one can encounter during a proteomic experiment, including, but not limited to sample preparation, sample fractionation, sample analysis, data analysis & interpretation and biological significance. Here we discuss some of the problems that researchers should be aware of when performing a proteomic experiment.
引用
收藏
页码：753 / 769
页数：17
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