Assembly of live micro-organisms on microstructured PDMS stamps by convective/capillary deposition for AFM bio-experiments

被引：48

作者：

Dague, E. ^{[1
,2
,3
]}

Jauvert, E. ^{[1
,2
,3
]}

Laplatine, L. ^{[1
,2
,3
]}

Viallet, B. ^{[4
]}

Thibault, C. ^{[1
,2
,3
]}

Ressier, L. ^{[4
]}

机构：

[1] CNRS, LAAS, F-31077 Toulouse 4, France

[2] CNRS, ITAV UMS3039, F-31106 Toulouse, France

[3] Univ Toulouse, UPS, INSA, INP,ISAE,UT1,UTM,LAAS,ITAV, Toulouse, France

[4] Univ Toulouse, INSA CNRS UPS, LPCNO, F-31077 Toulouse, France

来源：

NANOTECHNOLOGY | 2011年 / 22卷 / 39期

关键词：

ATOMIC-FORCE MICROSCOPY; HYDROPHOBIC RECOVERY; BACTERIA; POLY(DIMETHYLSILOXANE); RECOGNITION; PROTEINS; ADHESION;

D O I：

10.1088/0957-4484/22/39/395102

中图分类号：

TB3 [工程材料学];

学科分类号：

0805 ; 080502 ;

摘要：

Immobilization of live micro-organisms on solid substrates is an important prerequisite for atomic force microscopy (AFM) bio-experiments. The method employed must immobilize the cells firmly enough to enable them to withstand the lateral friction forces exerted by the tip during scanning but without denaturing the cell interface. In this work, a generic method for the assembly of living cells on specific areas of substrates is proposed. It consists in assembling the living cells within the patterns of microstructured, functionalized poly-dimethylsiloxane (PDMS) stamps using convective/capillary deposition. This versatile approach is validated by applying it to two systems of foremost importance in biotechnology and medicine: Saccharomyces cerevisiae yeasts and Aspergillus fumigatus fungal spores. We show that this method allows multiplexing AFM nanomechanical measurements by force spectroscopy on S. cerevisiae yeasts and high-resolution AFM imaging of germinated Aspergillus conidia in buffer medium. These two examples clearly demonstrate the immense potential of micro-organism assembly on functionalized, microstructured PDMS stamps by convective/capillary deposition for performing rigorous AFM bio-experiments on living cells.

引用

页数：7