Structural characterization of an immunoenhancing heteroglycan isolated from an aqueous extract of an edible mushroom, Lentinus squarrosulus (Mont.) Singer

被引：45
|
作者：
Bhunia, Sanjoy Kumar [1 ]
Dey, Biswajit [1 ]
Maity, Kankan K. [1 ]
Patra, Sukesh [1 ]
Mandal, Soumitra [1 ]
Maiti, Swatilekha [2 ]
Maiti, Tapas K. [2 ]
Sikdar, Samir R. [3 ]
Islam, Syed S. [1 ]
机构：
[1] Vidyasagar Univ, Dept Chem & Chem Technol, Midnapore 721102, West Bengal, India
[2] Indian Inst Technol IIT Kharagpur, Dept Biotechnol, Kharagpur 721302, West Bengal, India
[3] Bose Inst, Plant Mol & Cellular Genet Sect, Kolkata 700054, West Bengal, India
来源：
CARBOHYDRATE RESEARCH | 2010年 / 345卷 / 17期
关键词：
Lentinus squarrosulus (Mont.) Singer; Polysaccharide; Structure; NMR spectroscopy; Splenocyte and thymocyte activation; WATER-SOLUBLE GLUCAN; ASTRAEUS-HYGROMETRICUS; PLEUROTUS-FLORIDA; FRUIT-BODIES; (1->3)-BETA-D-GLUCAN; ELUCIDATION; ANTITUMOR;
D O I：
10.1016/j.carres.2010.09.015
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A water-soluble polysaccharide, isolated from the hot water extract of the fruiting bodies of an edible mushroom. Lentinus squarrosulus (Mont.) Singer was found to contain D-galactose, L-fucose, and D-glucose in a molar ratio of nearly 1:1:5. Structural investigation of the polysaccharide was carried out using acid hydrolysis, methylation analysis, periodate oxidation study, and NMR experiments (H-1, C-13, DQF-COSY, TOCSY, NOESY, ROESY, HMQC, and HMBC). On the basis of above-mentioned experiments, the structure of the repeating unit of the polysaccharide was established as: [GRAPHICS] This polysaccharide showed macrophage as well as splenocyte and thymocyte activation. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码：2542 / 2549
页数：8
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