Developing cryopreservation for Picea sitchensis (Sitka spruce) somatic embryos:: A comparison of vitrification protocols

被引:0
|
作者
Gale, Samantha [1 ,2 ]
John, Allan [2 ]
Harding, Keith [1 ]
Benson, Erica. E. [1 ]
机构
[1] Univ Abertay Dundee, Plant Conservat Grp, Dundee DD1 1HG, Scotland
[2] Forestry Commiss Roslin, No Res Stn, Roslin EH25 9SY, Midlothian, Scotland
关键词
clonal forestry; somatic embryos; vitrification; cryobanking;
D O I
暂无
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Two vitrification-based cryopreservation protocols, encapsulation/dehydration and PVS2 were applied to Stage 2 (globular) and Stage 4 (torpedo) somatic embryos (SE) from Picea sitchensis. Two recovery responses: partially differentiated embryogenic suspensor masses (ESM) and dedifferentiated non-embryogenic masses (NEM) were observed following exposure to LN. All genotypes tested, proliferated NEM, similar to 10 to 100% of the total SE cryopreserved. A General Linear Model applied to NEM recovery data demonstrated several different factors (developmental state and genotype, treatment, culture age) interacted at a significant level (P<0.05) to influence proliferation. One genotype was capable of proliferating ESM after cryopreservation using encapsulation/dehydration, this response was achieved for Stage 4 embryos derived from the youngest ESM tissue.
引用
收藏
页码:135 / 144
页数:10
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